PMID: 2492151Jan 1, 1989Paper

Effect of amino acid deprivation on initiation of protein synthesis in rat hepatocytes

The American Journal of Physiology
W V EversonL S Jefferson

Abstract

Conditions were defined for maintaining optimal protein synthetic activity in suspensions of freshly isolated rat hepatocytes. Under these conditions, isolated hepatocytes exhibited rates of protein synthesis and levels of polysomal aggregation equivalent to those observed in vivo and in perfused liver. Deprivation of total amino acids or single, essential amino acids resulted in a rapid decrease in the rate of protein synthesis, which was readily reversed by readdition of the deficient amino acid(s). The decrease was accompanied by a disaggregation of polysomes and an inhibition of 43S initiation complex formation, which was indicative of a limitation in the rate of initiation of protein synthesis. Extracts prepared from perfused liver deprived of amino acids were inhibitory to initiation of protein synthesis in reticulocyte lysate. The inhibition in reticulocyte lysate was accompanied by an increase in phosphorylation of the alpha-subunit of eukaryotic initiation factor 2 (eIF-2), suggesting activation of an eIF-2 alpha kinase or inhibition of a phosphatase in amino acid-deprived hepatocytes. This suggestion was confirmed by prelabeling hepatocytes with 32Pi before amino acid deprivation. Incorporation of 32Pi into eIF-2 alph...Continue Reading

References

Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J DelaunayI M London
Feb 15, 1979·The Biochemical Journal·M A McNurlanP J Garlick
Jan 24, 1977·Biochimica Et Biophysica Acta·P O Seglen
May 1, 1986·The Biochemical Journal·V M Pain
Jan 1, 1985·Annual Review of Biochemistry·K Moldave
Mar 1, 1980·Analytical Biochemistry·C Labarca, K Paigen
Jan 1, 1981·Progress in Nucleic Acid Research and Molecular Biology·R JagusB Safer

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Citations

Oct 6, 2017·Advances in Physiology Education·Virginie BourlierBénédicte Buffin-Meyer
May 1, 1990·The British Journal of Nutrition·D N SalterR J Elliott
Aug 14, 2001·American Journal of Physiology. Endocrinology and Metabolism·T G AnthonyL S Jefferson
Nov 15, 1996·The Journal of Biological Chemistry·K CianfloneA D Sniderman
Nov 30, 2000·American Journal of Physiology. Endocrinology and Metabolism·T A DavisS R Kimball
May 10, 2002·Nutritional Neuroscience·Linda J MagrumDorothy W Gietzen
Nov 24, 1999·The American Journal of Physiology·C H LangS R Kimball
Dec 23, 2008·Journal of Animal Science·K C DivakalaR L Payne
Sep 24, 2005·Biotechnology and Bioengineering·N S SharmaM L Yarmush
Aug 1, 2020·Cellular and Molecular Gastroenterology and Hepatology·Maria Dolors SansJohn A Williams
Dec 17, 1997·Biochemical and Biophysical Research Communications·F YoshizawaL S Jefferson
Mar 1, 1996·The International Journal of Biochemistry & Cell Biology·S R KimballL S Jefferson
Jun 24, 1998·Biochimica Et Biophysica Acta·E M Kurowska, K K Carroll
Jan 1, 1991·Pharmacology & Therapeutics·S J Morley, G Thomas
Apr 20, 1994·Biochimica Et Biophysica Acta·M S Medow, M S Lipkowitz
Jun 11, 2003·The Journal of Experimental Biology·Julian L PakayMichael Guppy
Sep 1, 1995·Metabolism: Clinical and Experimental·C JurasinskiT C Vary
Dec 1, 1996·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·R G HutsonM S Kilberg
Oct 1, 1996·Baillière's Clinical Endocrinology and Metabolism·K Smith, M J Rennie
Dec 31, 1990·Biochemical and Biophysical Research Communications·A R BaydounG E Mann
Dec 1, 1989·Current Opinion in Cell Biology·M J Clemens

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