Effect of amino acids on goat cauda epididymal sperm cryopreservation using a chemically defined model system

Cryobiology
C N KunduG C Majumder

Abstract

Studies were carried out to analyze the cryoprotecting efficacy of several amino acids by use of a chemically defined synthetic medium (modified Ringer's solution) and goat cauda epididymal sperm as the model system. Motile goat cauda sperm dispersed in the synthetic medium were subjected to a freezing protocol in a computer-controlled bio-freezer, cooling 0.25 degrees C x min(-1) to 5 degrees C, 5 degrees C x min(-1) to -20 degrees C, and 20 degrees C x min(-1) to -100 degrees C, prior to being plunged into liquid nitrogen. In the absence of amino acids, sperm cells completely lost their flagellar motility. Of all the amino acids tested, l-alanine showed maximal cryoprotection potential. l-Alanine at 135 mM offered optimum cryoprotection potential: recovery of sperm forward motility and total motility were 14 +/- 2% and 19 +/- 2%, respectively. l-Glutamine, l-proline, and glycine at optimum concentration (100-150 mM) cryopreserved approx. 11-17% total motility of the sperm cells, whereas amino acids such as l-arginine, l-lysine, and l-histidine offered little cryoprotection (0-5%) to the cells. Increasing the amino acid concentration beyond the optimum level sharply decreased the recovery of the sperm motility, which therefore...Continue Reading

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Citations

Apr 13, 2012·Physiological and Biochemical Zoology : PBZ·Annegret NicolaiMaryvonne Charrier
Oct 5, 2010·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Afzal R MohammedYvonne Perrie
Oct 31, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Chengjun ChenXing Tang
Jan 3, 2009·Animal Reproduction Science·E de MercadoJ Roca
Jan 18, 2007·Cryobiology·Jitamanyu ChakrabartyG C Majumder
Feb 24, 2007·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Afzal R MohammedYvonne Perrie
Jan 9, 2008·Reproduction in Domestic Animals = Zuchthygiene·M Z Ali Al AhmadF Fieni
Mar 30, 2010·Reproduction in Domestic Animals = Zuchthygiene·C YamadaJ A Visintin
Aug 12, 2016·Animal Reproduction Science·Hong-Jun DongYan Lin
Jul 13, 2020·Reproduction in Domestic Animals = Zuchthygiene·Parisa NazariJalal Rostamzadeh
Jan 30, 2020·Frontiers in Cell and Developmental Biology·Muhammet Rasit UgurErdogan Memili
Aug 14, 2020·International Journal of Molecular Sciences·Julian Santiago-Moreno, Elisabeth Blesbois

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