Abstract
(1) Background: For any antibacterial oral formulation to be successful, it must present effects in the presence of biofilms. Therefore, our aim is to analyze the drug release and the antibiofilm effects of a semi-solid formulation containing chlorhexidine (CHX) in the presence of pathogenic biofilms. (2) Methods: The biofilms of Streptococcus mutans (n = 6) or Porphyromonas gingivalis (n = 3) were formed for 6 and 4 days, respectively, being exposed to: 1) a CHX system or 2) vehicle control without CHX. A group without treatment was included as negative control. The acidogenicity, CHX quantification and bacterial viability were determined. A dissolution assay in a buffer and culture medium in the absence of bacteria was also performed. (3) Results: Although the CHX quantification in the culture medium of both biofilms was lower compared to the buffer (p < 0.05) and the culture medium in the absence of bacteria, the CHX system was able to display antibiofilm effects until 96 h for the S. mutans biofilms (p < 0.05) and 72 h for the P. gingivalis biofilms (p < 0.05). (4) Conclusions: The experimental formulation is able to extend chlorhexidine effects, even in challenging conditions such as in the presence of bacteria, allowing t...Continue Reading
References
Mar 1, 1994·Journal of Dental Research·J van Houte
Feb 13, 2001·Journal of Microbiological Methods·B HerigstadJ Heersink
Mar 21, 2001·Antimicrobial Agents and Chemotherapy·K Lewis
Mar 26, 2003·Oral Microbiology and Immunology·N Takahashi
Oct 18, 2003·The Journal of Antimicrobial Chemotherapy·H KooW H Bowen
Apr 16, 2005·Drug Development and Industrial Pharmacy·Marcos Luciano Bruschi, Osvaldo de Freitas
Sep 11, 2008·Caries Research·C P AiresJ A Cury
Jul 27, 2010·Brazilian Oral Research·Renzo Alberto Ccahuana-Vásquez, Jaime Aparecido Cury
Dec 8, 2010·Periodontology 2000·Thomas Beikler, Thomas F Flemmig
Mar 16, 2011·Oral Diseases·V SankarM Thornhill
Feb 9, 2012·International Journal of Dentistry·William PapaioannouMargarita Makou
Jan 18, 2013·Brazilian Oral Research·Sigmar de Mello RodeRui Vicente Oppermann
Jul 24, 2014·Brazilian Oral Research·Alex Nogueira HaasRui Vicente Oppermann
May 30, 2015·Archives of Oral Biology·V QuintasI Tomás
May 20, 2016·Marine Drugs·Barbara BellichAttilio Cesàro
Jun 29, 2016·European Journal of Oral Sciences·Dae-Woo LeeJae-Gyu Jeon
Sep 20, 2016·Biofouling·A C S RéC P Aires
Nov 4, 2017·Trends in Microbiology·William H BowenHyun Koo
Mar 8, 2018·International Journal of Dental Hygiene·T A ElkerboutG A Van der Weijden