Effect of an interferon-stimulated response element (ISRE) mutant of porcine circovirus type 2 (PCV2) on PCV2-induced pathological lesions in a porcine reproductive and respiratory syndrome virus (PRRSV) co-infection model

Veterinary Microbiology
Sheela RamamoorthyX J Meng

Abstract

Porcine circovirus type 2 (PCV2) is the primary causative agent of porcine circovirus-associated diseases (PCVAD) in swine. Coinfections of PCV2 with other swine pathogens increase the severity of PCVAD. Induction of proinflammatory cytokines by coinfecting pathogens may attribute to the exacerbation of PCVAD during coinfections. An interferon-stimulated response element (ISRE) sequence was identified in the origin of replication of PCV2 genome. To assess the role of ISRE in PCV2 pathogenesis during coinfection, an ISRE-mutant PCV2 was constructed and used to experimentally infect pigs with either ISRE mutant or wildtype PCV2 singly or in combination with porcine reproductive and respiratory syndrome virus (PRRSV). The results showed that, during early stage of infection at 14 days post-inoculation (dpi), the ISRE mutation reduced viral replication and elicited low antibody responses. However, at 28 dpi viremia in pigs infected with the ISRE-mutant was on an upward trend, and microscopic lesion scores in pigs inoculated with the ISRE-mutant were significantly more severe than in wildtype PCV2-infected pigs. Coinfection with PRRSV caused an opposite shift in the in vivo dynamics of the ISRE-mutant at 14 dpi with the lymph node h...Continue Reading

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Citations

Jul 12, 2014·Virus Genes·Qi-Zhuang LvYan-Ming Zhang
Jan 10, 2012·Veterinary Microbiology·Lavanya RammohanSheela Ramamoorthy
Oct 1, 2011·Virus Research·Tanja Opriessnig, Patrick G Halbur
Dec 19, 2015·Research in Veterinary Science·M A SsemadaaliS Ramamoorthy
Jan 10, 2012·Transboundary and Emerging Diseases·X J Meng
Jan 1, 2013·Annual Review of Animal Biosciences·Xiang-Jin Meng
Sep 5, 2015·Bioinformatics and Biology Insights·Megan ConstansSheela Ramamoorthy
Jan 19, 2012·Transboundary and Emerging Diseases·T Kekarainen, J Segalés
Jul 26, 2019·Transboundary and Emerging Diseases·Chunhua WeiJiankui Liu
Jun 18, 2020·Veterinary Research·Georges SaadeFrançois Meurens
Jul 22, 2021·Transboundary and Emerging Diseases·Agm Rakibuzzaman, Sheela Ramamoorthy

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