Effect of anandamide on cytosolic Ca(2+) levels and proliferation in canine renal tubular cells

Basic & Clinical Pharmacology & Toxicology
Jeng-Hsien YehChung-Ren Jan

Abstract

The effect of the endogenous cannabinoid anandamide on cytosolic free Ca(2+) concentration ([Ca(2+)](i)) and proliferation is largely unknown. This study examined whether anandamide altered Ca(2+) levels and caused Ca(2+)-dependent cell death in Madin-Darby canine kidney (MDCK) cells. [Ca(2+)](i) and cell death were measured using the fluorescent dyes fura-2 and WST-1 respectively. Anandamide at concentrations above 5 muM increased [Ca(2+)](i) in a concentration-dependent manner. The Ca(2+) signal was reduced by 78% by removing extracellular Ca(2+). The anandamide-induced Ca(2+) influx was insensitive to L-type Ca(2+) channel blockers and the cannabinoid receptor antagonist AM 251, but was inhibited differently by aristolochic acid, WIN 55,212-2 (a cannabinoid receptor agonist), phorbol ester, GF 109203X and forskolin. After pretreatment with thapsigargin (an endoplasmic reticulum Ca(2+) pump inhibitor), anandamide-induced Ca(2+) release was inhibited. Inhibition of phospholipase C with U73122 did not change anandamide-induced Ca(2+) release. At concentrations of 100 muM and 200 muM, anandamide killed 50% and 95% cells, respectively. The cytotoxic effect of 100 muM anandamide was completely reversed by pre-chelating cytosolic C...Continue Reading

References

Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·O ThastrupA P Dawson
Oct 1, 1995·Kidney International·F Lang, M Paulmichl
Sep 18, 1997·The Journal of Clinical Investigation·D G DeutschL C Moore
Aug 26, 1998·Biochemical and Biophysical Research Communications·J MatuszykL Strzadala
Oct 6, 1998·European Journal of Pharmacology·C R JanC J Tseng
Sep 15, 1999·Molecular and Cellular Biochemistry·S DasS Majumdar
Dec 3, 1999·Toxicology and Applied Pharmacology·J K Huang, C R Jan
Dec 7, 2000·Chemistry and Physics of Lipids·A C Howlett, S Mukhopadhyay
Jan 23, 2002·Pharmacology·Wei-Chuan ChenChung-Ren Jan
Jun 8, 2002·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Vincenzo Di MarzoT Bisogno
Aug 17, 2002·British Journal of Pharmacology·Manabu KagayaDomenico Spina
Jun 19, 2003·The Journal of Biological Chemistry·Andres A Caro, Arthur I Cederbaum
Aug 23, 2003·Cell Death and Differentiation·M Maccarrone, A Finazzi-Agró
Oct 1, 2003·British Journal of Pharmacology·Ruth A Ross
Dec 3, 2003·British Journal of Pharmacology·Cristina ZorattiWolfgang F Graier
May 22, 2004·Journal of the American Society of Nephrology : JASN·Yukako KouraTakao Saruta
Feb 16, 2005·Annual Review of Physiology·Michael J Berridge
Apr 21, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Sören V SiegmundRobert F Schwabe
Jun 1, 2005·Hearing Research·Sigrid E Myrdal, Peter S Steyger

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Citations

Jul 21, 2011·Drug and Chemical Toxicology·Chun-Chi KuoChung-Ren Jan
Nov 4, 2009·Journal of Receptor and Signal Transduction Research·Chun-Jen HuangChung-Ren Jan
Sep 20, 2008·Basic & Clinical Pharmacology & Toxicology·Wei-Chuan ChenChung-Ren Jan
Mar 31, 2012·Basic & Clinical Pharmacology & Toxicology·He-Hsiung ChengChung-Ren Jan
Nov 10, 2012·Journal of Receptor and Signal Transduction Research·Chung-Pin LiuChung-Ren Jan
Aug 22, 2008·Human & Experimental Toxicology·S I LiuC R Jan
Sep 23, 2009·Human & Experimental Toxicology·Yi-Chien FangChung-Ren Jan
Oct 17, 2017·Journal of the American Society of Nephrology : JASN·Liad HindenJoseph Tam
Aug 8, 2007·The Journal of Pharmacology and Experimental Therapeutics·Murat OzToni S Shippenberg
Apr 25, 2013·Human & Experimental Toxicology·Y-C FangC-R Jan

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