Abstract
Angiotensin II (AII), a product of rennin-angiotensin system, exerts an important role on the function of immune system cells. In this study, the effect of AII on the phagocytic activity of mouse peritoneal macrophages was assessed. Mice peritoneal macrophages were cultured for 48 h and the influence of different concentrations of AII (10(-14) to 10(-7) M) and/or losartan, 10(-16) to 10(-6) M), an AT1 angiotensin receptor antagonist, on phagocytic activity and superoxide anion production was determined. Dimethylthiazoldiphenyltetrazolium bromide reduction and the nucleic acid content were used to assess the cvtotoxicity of losartan. A stimulatory effect on phagocytic activity (P < 0.05) was observed with 10(-13) M and 10(-12 M) AII concentrations. The addition of losartan (up to10(-14) M) to the cell cultures blocked (P < 0.001) the phagocytosis indicating the involvement of AT1 receptors. In contrast, superoxide anion production was not affected by AII or losartan. The existence of AT1 and AT2 receptors in peritoneal macrophages was demonstrated by immunofluorescence microscopy. These results support the hypothesis that AII receptors can modulate murine macrophage activity and phagocytosis, and suggest that AII may have a ther...Continue Reading
References
Sep 1, 1990·Prostaglandins, Leukotrienes, and Essential Fatty Acids·P S PrabhaG Ramesh
Jul 1, 1987·Cellular Immunology·J V Weinstock, A M Blum
May 22, 1986·Journal of Immunological Methods·F Denizot, R Lang
Jan 1, 1986·Methods in Enzymology·D R Absolom
Jan 1, 1981·Journal of Immunological Methods·E Pick, D Mizel
Nov 1, 1984·Cellular Immunology·J V Weinstock, J T Kassab
Jan 1, 1993·Free Radical Research Communications·K V Kumar, U N Das
Mar 1, 1996·Hypertension·N IwaiM Kinoshita
May 1, 1996·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·V Regitz-ZagrosekE Fleck
Jan 1, 1997·Annual Review of Physiology·T Matsusaka, I Ichikawa
Mar 1, 1997·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·G NickenigA Sachinidis
Aug 22, 1997·The Journal of Biological Chemistry·K J ScheideggerJ L Witztum
Feb 12, 1998·The American Journal of Physiology·M E PueyoJ B Michel
Mar 10, 1998·Journal of Cellular Physiology·S LamparterJ Pfeilschifter
Sep 12, 1998·Kidney International·E A JaimesL Raij
Feb 4, 1999·Hypertension·Y YanagitaniT Ogihara
Jul 15, 1999·Journal of Hypertension·A OkamuraT Ogihara
Oct 23, 2002·The American Journal of Tropical Medicine and Hygiene·Patrícia Aline Boer-LimaMaria Alice Cruz-Höfling
May 15, 2003·Endocrinology·Julie L Lavoie, Curt D Sigmund
Jul 16, 2003·The American Journal of the Medical Sciences·Ursula C BrewsterMark A Perazella
Jan 1, 1991·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M R CingiF Zucco
Citations
Dec 21, 2006·International Immunopharmacology·Adel A GomaaEsraa A Ahmed
Mar 23, 2017·Inflammation·Sahin SultanaBiswadev Bishayi
Jun 28, 2016·Molecular Reproduction and Development·Mohamed Ali MareyAkio Miyamoto
Dec 9, 2017·Immunologic Research·Sahin SultanaBiswadev Bishayi
Dec 12, 2019·Immunobiology·Sahin Sultana, Biswadev Bishayi
Feb 8, 2020·Immunology Letters·Sahin Sultana, Biswadev Bishayi
Mar 30, 2019·Immunology Letters·Sahin SultanaBiswadev Bishayi
Jul 13, 2021·Frontiers in Cellular Neuroscience·Adonis SferaZisis Kozlakidis