Effect of angiotensin II and losartan on the phagocytic activity of peritoneal macrophages from Balb/C mice

Memórias do Instituto Oswaldo Cruz
Paula BellineJosé Francisco Figueiredo

Abstract

Angiotensin II (AII), a product of rennin-angiotensin system, exerts an important role on the function of immune system cells. In this study, the effect of AII on the phagocytic activity of mouse peritoneal macrophages was assessed. Mice peritoneal macrophages were cultured for 48 h and the influence of different concentrations of AII (10(-14) to 10(-7) M) and/or losartan, 10(-16) to 10(-6) M), an AT1 angiotensin receptor antagonist, on phagocytic activity and superoxide anion production was determined. Dimethylthiazoldiphenyltetrazolium bromide reduction and the nucleic acid content were used to assess the cvtotoxicity of losartan. A stimulatory effect on phagocytic activity (P < 0.05) was observed with 10(-13) M and 10(-12 M) AII concentrations. The addition of losartan (up to10(-14) M) to the cell cultures blocked (P < 0.001) the phagocytosis indicating the involvement of AT1 receptors. In contrast, superoxide anion production was not affected by AII or losartan. The existence of AT1 and AT2 receptors in peritoneal macrophages was demonstrated by immunofluorescence microscopy. These results support the hypothesis that AII receptors can modulate murine macrophage activity and phagocytosis, and suggest that AII may have a ther...Continue Reading

References

Sep 1, 1990·Prostaglandins, Leukotrienes, and Essential Fatty Acids·P S PrabhaG Ramesh
Jan 1, 1986·Methods in Enzymology·D R Absolom
Jan 1, 1993·Free Radical Research Communications·K V Kumar, U N Das
Mar 1, 1996·Hypertension·N IwaiM Kinoshita
May 1, 1996·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·V Regitz-ZagrosekE Fleck
Jan 1, 1997·Annual Review of Physiology·T Matsusaka, I Ichikawa
Mar 1, 1997·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·G NickenigA Sachinidis
Feb 12, 1998·The American Journal of Physiology·M E PueyoJ B Michel
Mar 10, 1998·Journal of Cellular Physiology·S LamparterJ Pfeilschifter
Sep 12, 1998·Kidney International·E A JaimesL Raij
Oct 23, 2002·The American Journal of Tropical Medicine and Hygiene·Patrícia Aline Boer-LimaMaria Alice Cruz-Höfling
May 15, 2003·Endocrinology·Julie L Lavoie, Curt D Sigmund
Jul 16, 2003·The American Journal of the Medical Sciences·Ursula C BrewsterMark A Perazella
Jan 1, 1991·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M R CingiF Zucco

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Citations

Dec 21, 2006·International Immunopharmacology·Adel A GomaaEsraa A Ahmed
Jun 28, 2016·Molecular Reproduction and Development·Mohamed Ali MareyAkio Miyamoto
Jul 13, 2021·Frontiers in Cellular Neuroscience·Adonis SferaZisis Kozlakidis

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