PMID: 8972391Dec 1, 1996Paper

Effect of angiotensin II on the apical K+ channel in the thick ascending limb of the rat kidney

The Journal of General Physiology
M LuW Wang

Abstract

We have used the patch-clamp technique to study the effect of angiotensin II (AII) on the activity of the apical 70 pS K+ channel and used Na(+)-sensitive fluorescent dye (SBFI) to investigate the effect of AII on intracellular Na+ concentration (Na+i) in the thick ascending limb (TAL) of the rat kidney. Addition of 50 pM AII reversibly reduced NPo, a product of channel open probability (Po) and channel number (N), to 40% of the control value and reduced the Na+i by 26%. The AII (50 pM)-induced decrease in channel activity defined by NPo was partially reversed by addition of 5 microM 17-octadecynoic acid (17-ODYA), an agent which blocks the cytochrome P450 monooxygenase. The notion that P450 metabolites of arachidonic acid (AA) may mediate the inhibitory effect of AII was further suggested by experiments in which addition of 10 nM of 20-hydroxyeicosatetraenoic acid (20-HETE) blocked the channel activity in cell-attached patches in the presence of 17-ODYA. We have used gas chromatography mass spectrometry (GC/MS) to measure the production of 20-HETE, a major AA metabolite of the P450-dependent pathway in the TAL of the rat. Addition of 50 pM AII increased the production of 20-HETE to 260% of the control value, indicating that 20...Continue Reading

Citations

Nov 1, 2003·Thrombosis Research·Nicholas R FerreriMairead A Carroll
Dec 15, 2000·Acta Physiologica Scandinavica·G Giebisch, W Wang
Jul 12, 2002·Kidney International·Jean-Stéphane PoumaratAnne Blanchard
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Oct 12, 2010·Journal of Cardiovascular Pharmacology·Jan M WilliamsRichard J Roman
Dec 13, 2005·American Journal of Physiology. Renal Physiology·Wen-Hui Wang
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