Effect of anionic lipids on ion permeation through the KcsA K+ -channel

Biochimica Et Biophysica Acta. Biomembranes
Victoria OakesCarmen Domene

Abstract

K+-channels are responsible for the efficient and selective conduction of K+ ions across the plasma membrane. The bacterial K+-channel KcsA has historically been used to characterize various aspects of K+ conduction via computational means. The energetic barriers associated with ion translocation across the KcsA selectivity filter have been computed in various studies, leading to the proposal of two alternate mechanisms of conduction, involving or neglecting the presence of water molecules in between the permeating ions. Here, the potential of mean force of K+ permeation is evaluated for KcsA in lipid bilayers containing anionic lipids, which is known to increase the open probability of the channel. In addition, the effect of the protonation/deprotonation of residue E71, which directly interacts with the selectivity filter sequence, is assessed. Both conduction mechanisms are considered throughout. The results obtained provide novel insights into the molecular functioning of K+-channels including the inactivation process.

References

Dec 1, 1993·Biophysical Journal·O S SmartB A Wallace
Apr 1, 1994·Biophysical Journal·L HeginbothamR MacKinnon
Feb 1, 1996·Journal of Molecular Graphics·W HumphreyK Schulten
Apr 29, 2000·Nature·J Aqvist, V Luzhkov
Nov 2, 2001·Nature·S Bernèche, B Roux
Jan 25, 2002·Biophysical Journal·Simon Bernèche, Benoît Roux
Mar 20, 2002·Biophysical Journal·Benoît Roux, Simon Bernèche
May 31, 2002·Nature·Youxing JiangRoderick MacKinnon
Aug 28, 2002·Biochemistry·Francis I ValiyaveetilRoderick MacKinnon
Sep 27, 2002·Biophysical Journal·Ian M WilliamsonAnthony G Lee
Apr 23, 2003·FEBS Letters·Jeroen A A DemmersJ Antoinette Killian
Dec 5, 2003·Journal of the American Chemical Society·Carmen DomeneMark S P Sansom
Nov 3, 2004·Biochimica Et Biophysica Acta·Anthony G Lee
Oct 14, 2005·Journal of Computational Chemistry·James C PhillipsKlaus Schulten
Nov 8, 2005·Biophysical Journal·Sundeep S DeolMark S P Sansom
Jan 22, 2008·Biochemistry·Francisco N BarreraJosé M González-Ros
Feb 26, 2008·Journal of the American Chemical Society·Carmen DomeneMichael L Klein
Mar 21, 2008·Journal of Computational Chemistry·Sunhwan JoWonpil Im
Apr 28, 2009·Journal of Molecular Biology·Carmen Domene, Simone Furini
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Simone Furini, Carmen Domene
May 26, 2010·The Journal of Physical Chemistry. B·Jeffery B KlaudaRichard W Pastor
Jul 9, 2010·Nature·Luis G CuelloEduardo Perozo
Aug 4, 2010·Proceedings of the National Academy of Sciences of the United States of America·Carmen Domene, Simone Furini
May 5, 2011·Journal of Molecular Biology·Simone Furini, Carmen Domene
Jan 1, 2009·Methods in Enzymology·Carmen Domene, Simone Furini
Oct 4, 2011·Biophysical Journal·Simone Furini, Carmen Domene
Dec 4, 2012·Biophysical Journal·Simone Furini, Carmen Domene
Dec 26, 2012·Proceedings of the National Academy of Sciences of the United States of America·Masayuki Iwamoto, Shigetoshi Oiki
Feb 22, 2013·Journal of the American Chemical Society·Markus WeingarthMarc Baldus

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Citations

Jul 30, 2021·Journal of the American Chemical Society·Carmen DomeneSimone Furini

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