Effect of Anoectochilus roxburghii flavonoids extract on H2O2 - Induced oxidative stress in LO2 cells and D-gal induced aging mice model.

Journal of Ethnopharmacology
Liping WangChunping Tang

Abstract

Anoectochilus roxburghii (A. roxburghii) is a popular folk medicine in many Asian countries, which has been used traditionally for treatment of some diseases such as diabetes, tumors, hyperlipemia, and hepatitis. The ethanol extract from A. roxburghii was recently shown to exert better ability to scavenge free radicals in vitro and possess antioxidant on natural aging mice in vivo. This study is to characterize the chemical composition, and investigate the protective effect of the A. roxburghii flavonoids extract (ARF) against hydrogen peroxide (H2O2)-induced oxidative stress in LO2 cells in vitro and D-galactose (D-gal)-induced aging mice model in vivo, and explore the underlying mechanisms. The chemical components of the flavonoids extract fromA. roxburghii were detected by ultraperformance lipid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UPLC-QTOF-MS/MS). H2O2 was used to establish an oxidative stress model in LO2 cells. Cytotoxic and protective effects of ARF on the LO2 cells were determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. Moreover, the levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and malondialdehyde (MDA) in cell supern...Continue Reading

Citations

Jul 30, 2020·Chemistry & Biodiversity·Cecilia DobreckyPablo Evelson
Sep 22, 2020·World Journal of Gastroenterology : WJG·Ronald Neil KostoffDarla Roye Shores
Feb 27, 2021·International Journal of Biological Macromolecules·Zheng ZhangYiqun Wan
Aug 14, 2021·Journal of Toxicology and Environmental Health. Part a·Doan Chinh ChungNghia Son Hoang

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