PMID: 6976305Jan 1, 1981Paper

Effect of anti-delta antibodies on clonal expansion and maturation of B lymphocytes

Immunobiology
L ForniA Coutinho

Abstract

The effect of a monoclonal anti-delta antibody on the in vitro B cell response to the B cell mitogen lipopolysaccharide (LPS) has been studied. Titration of anti-delta antibody in mass cultures stimulated by LPS resulted in a dose-dependent reduction of thymidine incorporation. IgM and IgG plaque forming cell (PFC) responses were poorly affected at high concentration, and slightly increased at low concentrations of anti-delta antibody. By limiting dilution analysis it was shown that anti-delta antibody inhibit 30-50% of LPS-reactive B cells to grow as IgM secreting clones, while increasing the average size of clones that grew in the presence of anti-delta as compared to control cultures. Anti-delta also results in increased frequencies of IgG secreting clones. By immunofluorescence it was possible to show the presence of a higher relative number of cells containing immunoglobulin as an effect of anti-delta antibodies. Observations made at early times of culture indicate that the cells that do not proliferate in the presence of anti-delta undergo an early maturation to secretion. Experiments performed on LPS blasts suggest that the effects of anti-delta on cell proliferation require the presence of antibodies at early times in t...Continue Reading

References

Mar 1, 1977·European Journal of Immunology·J L Preudhomme
Apr 1, 1978·The Journal of Experimental Medicine·S J BlackL A Herzenberg
Jun 1, 1978·The Journal of Experimental Medicine·J AnderssonF Melchers
Aug 1, 1976·European Journal of Immunology·E GronowiczF Melchers

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Citations

Apr 1, 1986·European Journal of Immunology·P PereiraA Coutinho
Sep 1, 1985·Scandinavian Journal of Immunology·L Forni
Apr 1, 1993·Immunological Reviews·E BaixerasC Martínez

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