PMID: 9433408Jan 1, 1997Paper

Effect of anti-DNA autoantibodies on the gene expression of interleukin 8, transforming growth factor-beta, and nitric oxide synthase in cultured endothelial cells

Scandinavian Journal of Rheumatology
K N LaiC K Lai

Abstract

Our study aims to determine whether anti-dsDNA exerts any effect on the gene expression of IL-8 or TGF-beta in cultured HUVEC. Both cytokines have angiogenic effect on endothelial cells. IgG was purified from 19 patients with SLE and from 19 healthy controls. Anti-dsDNA-depleted polyclonal IgG was also prepared from serum IgG of lupus patients by affinity chromatography with DNA cellulose column. Compared with either control IgG or anti-dsDNA-dep-IgG, HUVEC incubated with anti-dsDNA-containing-IgG expressed higher levels of IL-8 mRNA (p = 0.0001) and TGF-beta 1 mRNA (p = 0.0014). We demonstrated a significant increase in the percentage of cells with fragmented DNA in HUVEC incubated with anti-dsDNA-containing-IgG compared with those incubated with anti-dsDNA-dep-IgG, supporting the notion that anti-dsDNA may exert a direct apoptotic effect on cultured endothelial cells. Our study provides in vitro evidence that anti-dsDNA could play an important pathogenetic role in inducing inflammatory injury of vascular endothelium in SLE.

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Citations

Jul 5, 2012·Clinical & Developmental Immunology·Susan Yung, Tak Mao Chan
Oct 31, 2013·Clinical & Developmental Immunology·Susan YungTak Mao Chan
Apr 11, 2015·International Journal of Molecular Sciences·Desmond Y H Yap, Kar N Lai
Jun 29, 2006·Arthritis and Rheumatism·Xiaoping QingChaim Putterman
Mar 10, 2009·European Journal of Pharmacology·Hon-Pin WangBor-Show Tzang
Nov 1, 2007·Expert Review of Clinical Immunology·Mohammed Yousuf KarimDavid P D'Cruz
May 1, 2007·Arthritis and Rheumatism·Peter E WesterweelMarianne C Verhaar
May 10, 2005·Nephrology·Sydney TangKar Neng Lai
Oct 24, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dacheng DingMariana J Kaplan
Nov 22, 2001·Immunology Letters·A V KozyrN V Gnuchev

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