Effect of anti-gamma 3 antibodies on immunoglobulin isotype expression in lipopolysaccharide-stimulated cultures of mouse spleen cells

European Journal of Immunology
C F WebbM D Cooper

Abstract

To test the hypothesis that gamma 3 is the pivotal isotype for sequential heavy chain switching from mu to each of the gamma isotypes, we have compared the effects of anti-gamma 3 and anti-mu antibodies on the expression of immunoglobulin isotypes in lipopolysaccharide (LPS)-stimulated cultures of mouse spleen cells. IgM-, IgG1-, IgG2b- and IgG2a-containing plasma cells were enumerated by immunofluorescence and secreted immunoglobulins were measured by radioimmunoassay. Although anti-gamma 3 and anti-mu were equally effective in inhibiting the LPS-induced differentiation of IgG3 plasma cells, anti-gamma 3 had no effect on the differentiation of IgM, IgG1, IgG2b, or IgG2a plasma cells. These results support a direct mechanism of heavy chain immunoglobulin switching.

References

Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H HengartnerE Müller
Jul 1, 1976·European Journal of Immunology·K OkumuraL A Herzenberg
Jan 1, 1974·Contemporary Topics in Immunobiology·A R Lawton, M D Cooper
Nov 1, 1974·Scientific American·M D Cooper, A R Lawton
Dec 31, 1971·Annals of the New York Academy of Sciences·B PernisL Amante
Dec 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·P W KincadeM D Cooper
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·Y NishidaT Honjo
Jan 24, 1980·Nature·T H RabbittsD L Bentley
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·C ColecloughR P Perry
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R MakiS Tonegawa
Sep 19, 1980·Science·C P LiuF R Blattner
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P J GearhartJ J Cebra
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P D BurrowsM R Wabl
Jul 22, 1982·Nature·K B Marcu, M D Cooper
Oct 1, 1982·Journal of Clinical Immunology·H KubagawaM D Cooper
Oct 1, 1981·European Journal of Immunology·R BennerH Hooijkaas
Oct 1, 1963·The Biochemical Journal·F C GREENWOODJ S GLOVER

❮ Previous
Next ❯

Citations

Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C F WebbJ A Griffin
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·U Chen-BetteckenA Schimpl
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·S Haba, A Nisonoff
Jun 1, 1986·European Journal of Immunology·P PereiraA Coutinho
Dec 20, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·C F WebbE A Smith
Jul 1, 1992·European Journal of Immunology·G SiebenkottenA Radbruch
Mar 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·James S RushPhilip D Hodgkin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.