Effect of anti-mouse CD52 monoclonal antibody on mouse intestinal intraepithelial lymphocytes

Transplantation
Linlin QuJieshou Li

Abstract

CD52 monoclonal antibody (mAb) has been used therapeutically in lymphocytic leukemia, autoimmune disease, and organ transplantation. But the effect of CD52 mAb on intestinal intraepithelial lymphocytes (IELs) was unknown. The aim of this study was to assess the influence of anti-mouse CD52 mAb on IELs. Twenty male C57BL/6 mice were randomly assigned to a treatment or a control group. The treatment group received anti-mouse CD52 mAb (20 mug, subcutaneously), whereas the control group received the same volume of phosphate-buffered solution. On the seventh day after treatment, mice were killed, and ileum and colon were obtained for histopathology and immunohistochemistry examination, IELs were isolated for flow cytometric analysis and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide analysis. Furthermore, the intestinal permeability was measured by lactulose-mannitol test. The number and viability of IELs were decreased significantly in treatment compared with the control group. There were significant differences between the two groups considering IELs phenotypes. In addition, the proportion of apoptotic IELs in CD52 mAb treatment group was significantly higher than the control group (19.59%+/-3.13% and 2.02%+/-0.33%, ...Continue Reading

References

Jul 1, 1991·Annals of Clinical Biochemistry·J BloodP G Hill
Mar 1, 1990·Tissue Antigens·G HaleH Waldmann
Mar 17, 1995·The Journal of Biological Chemistry·A TreumannM A Ferguson
Jan 1, 1996·Molecular Human Reproduction·C Kirchhoff
Oct 9, 1999·The Journal of Biological Chemistry·S SchröterC Kirchhoff
Oct 19, 1999·Biochimica Et Biophysica Acta·M ToneH Waldmann
Jan 4, 2001·The Journal of Experimental Medicine·D DombrowiczM Capron
Oct 31, 2001·Nature Immunology·A HaydayJ Shires
Aug 27, 2003·Seminars in Oncology·Neus VillamorDolors Colomer
Sep 3, 2003·European Journal of Haematology·G A KennedyH M Prince
Dec 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·J Scott BrysonAlan M Kaplan
Jun 8, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Stanley I MartinLindsey R Baden
Jun 9, 2006·The New England Journal of Medicine·Santiago VivasDimas Suárez-Vilela
Jul 6, 2006·Annals of Hematology·Gaia GoteriItalo Bearzi
Aug 29, 2006·The American Journal of Pathology·Minoru HasegawaThomas F Tedder
Apr 10, 2007·Journal of Reproductive Immunology·K ItoK Koyama
Oct 3, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·R ShapiroT E Starzl
Apr 15, 2008·Microbes and Infection·Jun-ichi SakuragiTatsuo Shioda

❮ Previous
Next ❯

Citations

Apr 19, 2011·Cellular & Molecular Immunology·Qiurong LiJieshou Li
Oct 29, 2013·The British Journal of Nutrition·Honggang WangJieshou Li
Mar 23, 2015·The Journal of Surgical Research·Bo ShenXiujun Cai
Apr 17, 2020·Brain Sciences·Marina Kleopatra BozikiNikolaos Grigoriadis
Jun 4, 2018·Brain : a Journal of Neurology·Carlos R Camara-LemarroyV Wee Yong
Jan 31, 2021·Translational Research : the Journal of Laboratory and Clinical Medicine·Inessa CohenErin E Longbrake

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.