Effect of antibiotic supplementation on the quality of cryopreserved fish sperm of silver barb (Barbodes gonionotus): Sperm motility and viability, bacterial quality and fertilization

Animal Reproduction Science
Traimat BoonthaiVerapong Vuthiphandchai

Abstract

Disease prevention is a key aspect in developing cryopreservation procedures for fish sperm and in improving the reproductive biotechnology for commercially important aquatic species. The purpose of this study was to evaluate the effects of an antibiotic supplementation (0.25% penicillin-streptomycin, PS and 0.25% penicillin-gentamicin, PG) on sperm motility and viability, bacterial profile and fertilization capacity of cryopreserved silver barb (Barbodes gonionotus) semen. The experimental protocol involved three treatments: addition of PS alone; addition of PG alone; and no addition of antibiotics (Control). Semen samples were frozen and cryostored for 12-mo. Administration of 0.25% PS significantly (P<0.05) improved sperm motility and viability and reduced (P<0.05) total heterotrophic bacteria, Gram negative bacteria and pseudomonads bacteria in cryopreserved semen. Post-thawed semen treated with 0.25% PS did not contain contaminating bacteria including Bacillus subtilis subsp. inaquosorum, Bacillus safensis, Aeromonas punctata subsp. caviae, Serratia plymuthica, Pseudomonas azotoformans and Pseudomonas sp. Post-thawed semen supplemented with 0.25% PG showed degenerative changes in motility and viability of sperm. Eggs ferti...Continue Reading

References

Jul 15, 1995·Lancet·R S TedderK G Patterson
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Oct 26, 2005·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Benjamin BrennerElena Nizamoutdinova
Mar 30, 2006·Antimicrobial Agents and Chemotherapy·Hélène ServaisMarie-Paule Mingeot-Leclercq
Aug 15, 2006·TheScientificWorldJournal·Brian Austin
Jan 13, 2010·Clinical Microbiology Reviews·J Michael Janda, Sharon L Abbott
Dec 6, 2011·International Journal of Systematic and Evolutionary Microbiology·Ok-Sun KimJongsik Chun

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Related Papers

Schriftenreihe des Vereins für Wasser-, Boden- und Lufthygiene
R Bock
Veterinary Research Forum : an International Quarterly Journal
Ali Soleimanzadeh, Adel Saberivand
© 2022 Meta ULC. All rights reserved