Effect of antibiotic treatment on the formation of non-spore Clostridium difficile persister-like cells

The Journal of Antimicrobial Chemotherapy
R ÁlvarezF Gil

Abstract

The spore is the virulence factor identified to be involved in the recurrence of the disease caused by Clostridium difficile. To demonstrate that lethal antibiotic concentrations induce the appearance of C. difficile persister-like non-spore cells. C. difficile and derivative spo0A mutant strains were tested for their susceptibility to antibiotics, as determined using an agar dilution method. Persister-cell generation was determined for all strains using up to 10 × the MIC of every antibiotic for up to 6 days. Using up to 10 × the MIC of every antibiotic, we were able to induce the appearance of persister-like behaviour since biphasic killing curves could be observed in response to treatment antibiotics. To the best of our knowledge, this work provides, for the first time, experimental evidence of the appearance of C. difficile persister-like cells, opening a new research avenue in the pathogenesis of this nosocomial pathogen.

References

Jun 10, 2010·Annual Review of Microbiology·Kim Lewis
May 23, 2012·Infection and Immunity·Laura J DeakinTrevor D Lawley
Apr 27, 2013·The Journal of Antimicrobial Chemotherapy·Abhishek DeshpandeCurtis J Donskey
May 9, 2014·Future Microbiology·Jonathan Barra-Carrasco, Daniel Paredes-Sabja
Sep 4, 2014·Microbiology and Molecular Biology Reviews : MMBR·David LebeauxChristophe Beloin
Apr 30, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Charlesnika T Evans, Nasia Safdar

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