Effect of antimicrobial peptides on ATPase activity and proton pumping in plasma membrane vesicles obtained from mycobacteria

Peptides
Paola SantosCarlos-Yesid Soto

Abstract

The potential usefulness of antimicrobial peptides (AMPs) as antimycobacterial compounds has not been extensively explored. Although a myriad of studies on AMPs from different sources have been done, some of its mechanisms of action are still unknown. Maganins are of particular interest since they do not lyse non-dividing mammalian cells. In this work, AMPs with well-recognized activity against bacteria were synthesized, characterized, purified and their antimycobacterial activity and influence on ATPase activity in mycobacterial plasma membrane vesicles were assessed. Using bioinformatics tools, a magainin-I analog peptide (MIAP) with improved antimicrobial activity was designed. The influence of MIAP on proton (H(+)) pumping mediated by F(1)F(0)-ATPase in plasma membrane vesicles obtained from Mycobacterium tuberculosis was evaluated. We observed that the antimycobacterial activity of AMPs was low and variable. However, the activity of the designed peptide MIAP against M. tuberculosis was 2-fold higher in comparison to magainin-I. The basal ATPase activity of mycobacterial plasma membrane vesicles decreased approximately 24-30% in the presence of AMPs. On the other hand, the MIAP peptide completely abolished the F(1)F(0)-ATPa...Continue Reading

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Citations

May 15, 2015·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Andrés León-TorresCarlos-Y Soto
Jun 5, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·João P SilvaF Miguel Gama
Sep 14, 2019·Journal of Peptide Science : an Official Publication of the European Peptide Society·Sutharsana YathursanVijayalekshmi Sarojini
Sep 1, 2017·Applied Microbiology and Biotechnology·Richa SharmaIndu Verma
Jun 27, 2019·Current Protein & Peptide Science·Sara Silva, Nuno Vale
Nov 23, 2017·Frontiers in Immunology·Javier Arranz-TrullénEster Boix

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