Effect of Antimuscarinic Autoantibodies in Primary Sjögren's Syndrome

Journal of Dental Research
N KimKyungpyo Park

Abstract

The presence of functional autoantibodies against the muscarinic type 3 receptor (M3R) has been reported in primary Sjögren's syndrome (pSS). However, the pathogenic role of these autoantibodies in pSS development remains to be elucidated. In this experiment, we investigated a pathologic role of pSS autoantibodies (pSS IgG) associated with downregulation of the major histocompatibility complex I (MHC I) molecule with M3R through internalization. Anti-M3R autoantibodies in purified control and pSS IgG were detected using 4 synthesized cyclic M3R peptides by enzyme-linked immunosorbent assay. The binding reactivity of pSS IgG to M3R in situ was analyzed by a dual immunostaining method. Surface expression, interaction, and internalization of M3R with MHC I were analyzed by immunofluorescence confocal microscopy and biochemical assays. Synthetic cyclic peptides M3RP(205-221) and M3RP(520-527) showed significantly high reactivity with pSS IgG compared to the control IgG or the other 3 peptides (P < 0.05). Significantly high reactivity of pSS IgG to M3R in situ was observed. PSS IgG increased the interaction of membrane M3R with MHC I and induced their internalization in primary human submandibular gland cells. The pSS IgG-induced in...Continue Reading

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Citations

Feb 2, 2016·International Journal of Molecular Sciences·Christine DelporteJason Perret
Jul 3, 2016·Archives of Dermatological Research·Farzaneh Agha-HosseiniNeda Mohammadpour
Jul 8, 2020·Cells·Claudia D'AgostinoChristine Delporte
Jul 28, 2017·Molecular Immunology·Eun NamkoongKyungpyo Park

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Methods Mentioned

BETA
Enzyme-linked Immunosorbent
electrophoresis
ELISA
coimmunoprecipitation
immunoprecipitation

Software Mentioned

GenScript
ZEN

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