Effect of apoA-I on cholesterol release and apoE secretion in human mature adipocytes.

Lipids in Health and Disease
Karima BencharifRégis Roche

Abstract

The risk of cardiovascular disease is inversely correlated to level of plasma HDL-c. Moreover, reverse cholesterol transport (RCT) from peripheral tissues to the liver is the most widely accepted mechanism linked to the anti-atherosclerotic activity of HDL. The apolipoprotein A-I (apoA-I) and the ABC transporters play a key role in this process.Adipose tissue constitutes the body's largest pool of free cholesterol. The adipose cell could therefore be regarded as a key factor in cholesterol homeostasis. The present study investigates the capacity of primary cultures of mature human adipocytes to release cholesterol and explores the relationships between apoA-I, ABCA1, and apoE as well as the signaling pathways that could be potentially involved. We demonstrate that apoA-I induces a strong increase in cholesterol release and apoE secretion from adipocytes, whereas it has no transcriptional effect on ABCA1 or apoE genes. Furthermore, brefeldin A (BFA), an intracellular trafficking inhibitor, reduces basal cholesterol and apoE secretion, but does not modify induction by apoA-I. The use of statins also demonstrates that apoA-I stimulated cholesterol release is independent of HMG-CoA reductase activation. Our work highlights the fact...Continue Reading

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Citations

Jun 28, 2011·Journal of Lipid Research·Jianjia FanCheryl L Wellington
Mar 26, 2016·The Journal of Investigative Dermatology·Louisa CheungNing Xu Landén
Dec 20, 2011·Biochimica Et Biophysica Acta·Kimberly A EdgelRenée C Leboeuf
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Feb 7, 2013·Journal of Receptor and Signal Transduction Research·Tian-Biao Zhou
Oct 28, 2015·Journal of Lipid Research·Maria LindahlKarin G Stenkula
Nov 28, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xia GuoAbulizi Abudula
Feb 26, 2016·Lipids in Health and Disease·Shuai WangYuhong Yi
Mar 30, 2018·International Journal of Endocrinology·Sofia DiasLaura Ribeiro

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