PMID: 9003067Feb 1, 1997Paper

Effect of apolipoprotein E on neurite outgrowth and beta-amyloid-induced toxicity in developing rat primary hippocampal cultures

Journal of Neurochemistry
P S PuttfarckenM J LaDu

Abstract

The correlation between the epsilon 4 allele of apolipoprotein E (apoE) and Alzheimer's disease is well established. However, the role of apoE in normal as well as pathological brain processes remains unclear. We evaluated the effect of apoE treatment on development and beta-amyloid (A beta)-induced toxicity using primary cultures of developing rat hippocampal neurons. The source of apoE was conditioned media from HEK cells stably transfected with human apoE3 or apoE4 cDNA, a preparation where apoE is lipid-associated. Morphological and biochemical changes in the cultures were assessed at 1 and 3 days following low- and high-density plating with either apoE3 or E4 with or without A beta. Both apoE isoforms were neurotrophic, as measured by increased neurite length. Aged A beta(1-42), a peptide preparation exhibiting extensive fibril and aggregate formation, is toxic to these cultures. Addition of apoE3 and E4 significantly and comparably attenuated the A beta-induced reduction in both neurite length and cell viability. The level of protection against this toxicity was proportional to the neurotrophic actions of the two apoE isoforms. Thus, apoE acts as a potent growth factor in both the absence and the presence of A beta, suppo...Continue Reading

Citations

Aug 15, 2000·The Journal of Biological Chemistry·M J LaDuL J van Eldik
Apr 22, 2009·Journal of Neuroscience Research·Barbara LorberAnn Logan
Aug 28, 2013·Alzheimer's & Dementia : the Journal of the Alzheimer's Association·Swetha VijayaraghavanTaher Darreh-Shori
Oct 23, 2002·Journal of Neuroscience Research·Bruce Teter, J Wesson Ashford
May 19, 2000·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·P S Green, J W Simpkins
Jul 12, 2002·Journal of Neuroscience Research·B TeterG M Cole
Jan 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jean-Cosme DodartSteven M Paul
Nov 6, 2020·Molecular Neurodegeneration·Zonghua LiGuojun Bu
Oct 20, 1998·Journal of Neuroscience Research·M Michikawa, K Yanagisawa
Jan 1, 1997·Neurobiology of Disease·T WisniewskiB Frangione
Oct 19, 2006·Neurobiology of Aging·Bruce Teter
Aug 15, 2009·Neuron·Jungsu KimDavid M Holtzman

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