Effect of apolipoprotein E variants on lipolysis of very low density lipoproteins by heparan sulphate proteoglycan-bound lipoprotein lipase

Atherosclerosis
F H de ManL M Havekes

Abstract

Lipoprotein lipase (LPL) is bound to heparan sulphate proteoglycans (HSPG) at the luminal surface of endothelium. It is the key enzyme involved in the hydrolysis of very low density lipoproteins (VLDL). Prior to lipolysis by LPL, the lipoproteins are considered to interact with vessel wall HSPG. Apolipoprotein (apo) E is thought to mediate this interaction thereby enhancing the stability of the lipoprotein-LPL complex. We hypothesize that apo E mutations may cause a diminished interaction of VLDL with HSPG leading to impaired lipolysis of VLDL by HSPG-bound LPL. In order to test this hypothesis, lipolysis experiments were performed using HSPG-bound LPL. The mean lipolysis rates of VLDL, isolated from the apo E2 (Lys146-->Gln) heterozygotes, apo E2 (Arg158-->Cys) homozygotes and apo E3-Leiden heterozygotes were 92.3 +/- 10.3 (ns), 77.3 +/- 4.2 (P < 0.05) and 76.7 +/- 10.0% (P < 0.05), respectively, of that of control VLDL (100.0 +/- 9.7%). No differences in lipolysis were observed between VLDL from controls and VLDL from the same patients if LPL in solution was used. Thus, compositional differences alone can not explain the differences in lipolysis rates observed with HSPG-bound LPL. In competition experiments, the binding effic...Continue Reading

References

May 12, 1975·Analytical Biochemistry·T G RedgraveC E West
Sep 22, 1992·Biochimica Et Biophysica Acta·K MoriyamaK Arakawa
Sep 1, 1994·The Journal of Clinical Investigation·P de KnijffL M Havekes
Oct 1, 1995·Current Opinion in Lipidology·G Olivecrona, T Olivecrona

❮ Previous
Next ❯

Citations

Nov 13, 2003·Atherosclerosis·Katherine CianfloneBrian M Gilfix
Jun 5, 2003·Biochimica Et Biophysica Acta·Mayumi YamamotoTetsurou Handa
Jul 18, 2006·Atherosclerosis·Richard L SeipPaul D Thompson
Nov 22, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Julien A MenardMattias Belting
Apr 13, 2021·Frontiers in Oncology·Vasileios LazarisKyriakos E Kypreos
Jul 15, 2006·Metabolism: Clinical and Experimental·Masumi HaraKazuhisa Tsukamoto

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE Phenotypes

Apolipoprotein E (APOE) is a protein involved in fat metabolism and associated with the pathogenesis of Alzheimer's disease and cardiovascular disease. Here is the latest research on APOE phenotypes.