Effect of apoptosis in neural stem cells treated with sevoflurane

BMC Anesthesiology
Jianlei QiuYuelan Wang

Abstract

At present, sevoflurane inhalation anesthesia used on infants is well-known. But long-time exposure to inhalation anesthetic could cause neurologic disorder, especially nerve degeneration in infant and developing brain. The central nervous system degeneration of infants could affect the memory and cognitive function. γ-Aminobutyric acid (GABA) is a known inhibitory neurotransmitter in central nervous system. Inhalation anesthetic sevoflurane may activate GABAA receptor to inhibit central nervous system, leading to apoptosis of neural degeneration, cognitive dysfunction in the critical period of brain development. Neural stem cells were derived from Wistar embryos, cultured in vitro. Third generation of neural stem cells were randomly divided into four groups according to cultured suspension: Sevoflurane group (Group S), GABAA receptor antagonists, Bicuculline group (Group B), Sevoflurane + GABAA receptor antagonists, Bicuculline group (Group S + B), dimethyl sulphoxide (DMSO) group (Group D). Group B and Group D did not receive sevoflurane preconditioning. Group S and Group S + B were pretreated with 1 minimum alveolar concentration (MAC) sevoflurane for 0 h, 3 h, 6 h, and 12 h. Group S + B and Group B were pretreated with bicu...Continue Reading

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Citations

Sep 10, 2020·The International Journal of Neuroscience·Qirui WangJiekun Gong
Jul 26, 2018·Neurochemical Research·Mohammed A KashemVladimir J Balcar
Aug 15, 2020·Frontiers in Medicine·Lei ZhangHong Jiang

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Methods Mentioned

BETA
flow cytometry
immunoprecipitation assay
protein assay

Software Mentioned

Image J
SPSS

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