Effect of aqueous tobacco smoke extract and shear stress on PECAM-1 expression and cell motility in human uterine endothelial cells

Toxicological Sciences : an Official Journal of the Society of Toxicology
Arlen SoghomoniansGordon C Douglas

Abstract

Tobacco smoke constituents have several adverse effects on endothelial cells. Exposure to tobacco smoke during pregnancy is associated with adverse effects on pregnancy outcome possibly related to endothelial dysfunction. Platelet endothelial cell adhesion molecule-1 (PECAM-1) is an important regulator of endothelial function. This study tests the idea that an aqueous extract of cigarette smoke alters the expression of PECAM-1 in uterine endothelial cells. Human uterine microvascular endothelial cells were cultured in cigarette smoke-conditioned medium (CSM) under arterial physiological flow conditions (shear or frictional stress in the range 7.5-15 dyne/cm(2)) and the expression of PECAM-1 was assessed by immunofluorescence microscopy and Western blotting. Thick reticular PECAM-1-associated bands found at cell-cell junctions in static cultures became significantly thinner or disappeared when the cells were exposed to shear stress or to CSM for 24 h. This diminution at cell junctions was accompanied by increased punctate cytoplasmic/cell surface staining. Under shear stress conditions, PECAM-1 was equally distributed between cell surface and intracellular sites. In contrast, when cells were exposed to both shear stress and CSM,...Continue Reading

Citations

May 30, 2009·Analytical and Bioanalytical Chemistry·Jeffery S EdmistonWillie J McKinney
Aug 6, 2010·Human Reproduction Update·C DechanetH Dechaud
Jul 16, 2014·Expert Review of Proteomics·Sabika FirasatAbdul R Asif
Jan 16, 2010·Systems Biology in Reproductive Medicine·Jacqui Detmar, Andrea Jurisicova
Apr 3, 2008·Birth Defects Research. Part C, Embryo Today : Reviews·Prudence Talbot
Sep 7, 2019·Journal of Cellular Physiology·Jia LiuNanping Wang
Mar 14, 2020·Reproductive Biology and Endocrinology : RB&E·Cristina de AngelisRosario Pivonello

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