Effect of arachidonic and eicosapentaenoic acid metabolism on RAW 264.7 macrophage proliferation

Journal of Cellular Physiology
Diana Nieves, Juan José Moreno

Abstract

Prostaglandins (PGs) and leukotrienes (LTs) derived from arachidonic acid (AA) are potent mediators of inflammation and cell proliferation. Dietary intake of eicosapentaenoic acid (EPA) appears beneficial to both inflammatory processes and cell proliferation. However, there is no clear mechanism explaining these effects. In this study, we investigated the effect of EPA on the AA incorporation in phospholipid membranes, on AA release and metabolism, and consequently, on PG synthesis. Our results showed not only that [(3)H]AA and [(14)C]EPA were similar incorporated into RAW 264.7 macrophage membranes, but also that the redistribution pattern between phospholipids was alike. [(3)H]AA or [(14)C]EPA release was induced by fetal bovine serum (FBS) in a similar fashion with AA metabolizing 3-fold more than EPA. In this way, we observed that AA could be metabolized by cyclooxygenase (COX)-1, COX-2 and 5-lipoxygenase (5-LOX) whereas EPA was metabolized by COX-2 and 5-LOX pathways. Moreover, both fatty acids were able to induce COX-2 expression. When we incubated [(3)H]AA labeled cells with exogenous EPA, we observed that EPA did not modify FBS-induced [(3)H]AA release but that the presence of EPA decreased [(3)H]AA metabolism and there...Continue Reading

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Citations

Jul 2, 2008·The British Journal of Nutrition·Uta EnkeUdo R Markert
Apr 30, 2008·World Journal of Gastroenterology : WJG·Bo ZhangSheng Li
Sep 23, 2011·The Journal of Nutritional Biochemistry·Andrew P NeilsonDean E Brenner
Nov 9, 2010·Nutrition Research·K Shane BroughtonBruce Culver
Mar 26, 2011·Journal of Cellular Physiology·Hilton Kenji TakahashiRui Curi
Sep 20, 2006·Chemico-biological Interactions·Ze-Jian WangMing Yin
Jun 1, 2018·Pharmaceuticals·Henrike HornMarco Koch
Aug 18, 2017·Expert Opinion on Drug Discovery·Li MaAllan V Kalueff
Mar 31, 2019·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Jan KorbeckiMieczysław Dutka
Jul 15, 2020·Critical Reviews in Food Science and Nutrition·Ningning HuangHongkui Wei

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