Effect of arsenic and cadmium on the persistence of mutagen-induced DNA lesions in human cells

Environmental and Molecular Mutagenesis
A Hartmann, G Speit

Abstract

The alkaline single cell gel electrophoresis (SCG test or comet assay) was used to characterize the influence of sodium arsenite (NaAsO2) and cadmium sulphate (CdSO4) on the persistence of mutagen-induced DNA lesions. Human blood and SV4O-transformed fibroblasts (MRC5CV1) were treated for 2 hr with methyl methanesulphonate (MMS) or benzo(a)pyrene (BaP). MMS induced concentration-related DNA damage in white Blood cells (WBC) and fibroblasts in similar concentrations. For the induction of DNA damage in white blood cells (WBC) and fibroblasts in similar concentrations. For the induction of DNA damage by BaP, higher concentrations had to be applied to WBC than to the fibroblast cell line. To study the influence of metal ions on the persistence of DNA lesions, treated cells were further incubated for 2 hr in the absence (postincubation) or presence (posttreatment) of NaAsO2 or CdSO4. After postincubation, MMS and BaP-induced DNA effects were reduced in both cell types, indicating that repair of DNA lesions had taken place. When the cells were posttreated with NaAsO2 or CdSO4, BaP- and MMS-induced DNA lesions persisted in both cell types, indicating an inhibition of DNA repair by these metals. The results suggest a strong interaction...Continue Reading

References

Nov 1, 1977·Mutation Research·G D ZasukhinaZ S Kirkova
Dec 16, 1992·Mutation Research·A N JhaA T Natarajan
Sep 1, 1992·International Journal of Radiation Biology·C M GedikA R Collins
Jan 1, 1992·Critical Reviews in Toxicology·M P WaalkesR A Barter
Nov 1, 1990·Mutation Research·V M MaherJ J McCormick
Jul 1, 1989·Biological Trace Element Research·A Hartwig, D Beyersmann
Jul 1, 1989·Biological Trace Element Research·J H Li, T G Rossman
Mar 1, 1988·Experimental Cell Research·N P SinghE L Schneider
Dec 17, 1985·Biochimica Et Biophysica Acta·R SaffhillP J O'Connor
Jul 1, 1993·Mutation Research·V J McKelvey-MartinA Collins
Feb 1, 1995·Mutation Research·D W FairbairnK L O'Neill
Jun 1, 1992·Environmental Geochemistry and Health·M J Mass

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Citations

Jun 15, 2001·Teratogenesis, Carcinogenesis, and Mutagenesis·M SordoE Rojas
Dec 18, 2001·Teratogenesis, Carcinogenesis, and Mutagenesis·K D DeviP Grover
Jul 24, 2008·Fish Physiology and Biochemistry·Cheng WuFashui Hong
Apr 6, 2002·Mutation Research·D MenéndezP Ostrosky-Wegman
Jun 9, 2001·Biomaterials·D J Chauvel-LebretM Bonnaure-Mallet
Aug 14, 2003·Ecotoxicology and Environmental Safety·Jaqueline CalderónFernando Díaz-Barriga
Jun 18, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·T FaturM Filipic
Oct 24, 2002·Toxicology·Sentaro TakahashiRyuichi Okayasu
May 30, 2002·Mutation Research·Andrew MaierAlvaro Puga
May 22, 2003·International Immunopharmacology·Georgina GaliciaRafael Saavedra
Jun 29, 2011·Journal of Toxicology and Environmental Health. Part a·Vanessa ValdiglesiasBlanca Laffon
Aug 3, 2006·Environmental Health Perspectives·Angeline S AndrewMargaret R Karagas
Apr 22, 2009·Molecular Nutrition & Food Research·Maike NollenTanja Schwerdtle
Jul 14, 2017·International Journal of Molecular Sciences·Neenu SinghShareen H Doak
Feb 6, 2003·International Journal of Cancer. Journal International Du Cancer·Angeline S AndrewJoshua W Hamilton
Jul 15, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Yuonne KnöbelBeatrice Louise Pool-Zobel
Oct 14, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Joseph R ShawBruce A Stanton
Oct 1, 1996·Reviews on Environmental Health·B M HasspielerK Adeli
May 1, 1997·Toxicology and Applied Pharmacology·S LynnK Y Jan
Mar 6, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·E RojasM Valverde
Jan 18, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Y KnöbelB L Pool-Zobel

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