Effect of aspiration pressure during oocyte harvesting on oocyte recovery and in vitro development of ovine oocytes

Reproduction in Domestic Animals = Zuchthygiene
K M MortonG Evans

Abstract

Oocytes from abattoir-sourced ovine ovaries were aspirated from 2- to 4-mm follicles using 25, 50 or 100 mmHg pressure and an aspiration pump, or a needle (20-G) and syringe (2.5 ml) and subjected to in vitro maturation, fertilization and culture to determine the effect of aspiration pressure on the number and quality of oocytes recovered, and early embryonic development. Oocyte recovery rate was similar between groups (range: 57.1-73.1%; p > 0.05). The number and percentage of grade I and II oocytes recovered was reduced for 100 mm (24.5 +/- 3.6 and 31.1 +/- 6.1%) compared with 25 mm (51.4 +/- 7.0 and 60.2 +/- 7.8%) and 50 mm pressure (40.8 +/- 5.6 and 50.3 +/- 4.4%) and a syringe (40.3 +/- 12.0 and 45.2 +/- 2.1%; p < 0.05). Oocyte cleavage was similar for all groups at 24 (range: 30.9-49.6%) and 48 h post-insemination (49.7-65.5%), but blastocyst formation (% cleaved oocytes) was lower for oocytes aspirated with 25 mm (37.8%) than 50 (69.2%) or 100 mm (67.2%) pressure, and a syringe (72.0%; p < 0.05). Embryo production efficiency (% of oocytes cultured developing to the blastocyst stage) was higher for oocytes aspirated with 50 mm (45.4%) and 100 mm pressure (43.8%) and a syringe (45.0%) than 25 mm pressure (18.8%; p < 0.05)....Continue Reading

References

Nov 1, 1985·Journal of Reproduction and Fertility·R M Moor, I M Crosby
May 1, 1974·Journal of Reproduction and Fertility·H R Tervit, L E Rowson
Aug 1, 1997·Animal Reproduction Science·M Kafi, M R McGowan
Mar 29, 2000·Theriogenology·M StanglG Brem
Nov 6, 2003·The Journal of Veterinary Medical Science·Yoshihiko SasamotoYoshiyuki Takahashi
Sep 10, 2005·Reproduction in Domestic Animals = Zuchthygiene·K M MortonG Evans
Mar 8, 2006·Reproduction in Domestic Animals = Zuchthygiene·C RodríguezP de Paz
Apr 1, 1997·Theriogenology·R C FryJ Reynolds

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Citations

Sep 30, 2008·Pakistan Journal of Biological Sciences : PJBS·Chutichudet BenjawanS Khumkratok
Mar 8, 2019·Reproduction : the Official Journal of the Society for the Study of Fertility·T LeahyS P de Graaf
Jan 10, 2020·Reproduction in Domestic Animals = Zuchthygiene·Felipe Farias Pereira da Câmara BarrosWilter Ricardo Russiano Vicente

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