PMID: 8972705Dec 4, 1996Paper

Effect of astroglial cell swelling on pH of acidic intracellular compartments

Biochimica Et Biophysica Acta
G L BuschF Lang

Abstract

A variety of pathological conditions lead to swelling of astrocytes, which in turn stimulates ion release by activation of ion channels at the plasma membrane. In the present study, acridine orange and fluorescein isothiocyanate coupled to dextran (FITC-dextran) have been used to examine the effect of cell swelling on pH in acidic compartments of cultured astroglial cells. Both NH4Cl (2 mM) and chloroquine (10 microM), known to alkalinize acidic cellular compartments, led to the expected increase in acridine orange fluorescence intensity. Similar, albeit smaller, effects were elicited by a reduction of extracellular osmolarity (-80 mOsm) and treatment of the cells with glutamate (l mM), manoeuvres which enhanced cell volume. Determination of changes in the FITC-dextran fluorescence ratio (485/440 nm) allowed quantification of the pH changes in lysosomal compartments. Treatment with NH4Cl, reduced extracellular osmolarity and glutamate increased lysosomal pH by 0.65 +/- 0.07, 0.85 +/- 0.14 and 0.25 +/- 0.07, respectively. Measurement of cytosolic pH using 2',7',-bis-(2-carboxyethyl)-5- (and -6) carboxyfluorescein (BCECF) demonstrated a pronounced acidification following cell swelling, observed with both reduced extracellular osm...Continue Reading

References

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Citations

May 24, 2005·Neurochemistry International·Dieter Häussinger, Freimut Schliess
Dec 15, 2000·Progress in Neurobiology·J L VeneroJ Cano
Dec 11, 2002·Journal of Gastroenterology and Hepatology·Dieter HäussingerGerald Kircheis
Nov 4, 2006·Biological Chemistry·Freimut SchliessDieter Häussinger
Mar 20, 2010·Journal of Cellular Physiology·Johannes KornhuberStefan Trapp
May 3, 2005·Journal of Biomechanics·P Grace ChaoClark T Hung
Dec 18, 2001·Journal of Neuroscience Research·G BaiM D Norenberg
Jan 23, 1999·Bioelectromagnetics·Y YenC K Chou
May 27, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Dieter Häussinger
Feb 11, 1998·Physiological Reviews·F LangD Häussinger
Jan 31, 2002·World Journal of Gastroenterology : WJG·S vom DahlD Häussinger
Dec 31, 2005·Metabolic Brain Disease·Dieter HäussingerFreimut Schliess
May 4, 2000·American Journal of Physiology. Cell Physiology·R HanuR J Bloch
Nov 14, 1997·The American Journal of Physiology·A Krämer-GuthF Lang

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