Effect of ATP and platelet-activating factor on intracellular calcium concentrations of cultured oviductal cells from cows

Journal of Reproduction and Fertility
U TiemannM Spitschak

Abstract

Oviductal endosalpingeal cells were mechanically isolated from heifers at different reproductive stages (cyclic: days 8-14, at oestrus: day 0 and pregnant: day 7) and cultured until 100% confluent. The cells were loaded with the Ca(2+)-sensitive fluorochrome fura-2/AM, and cytosolic free calcium ([Ca2+]i) was monitored spectrofluorimetrically and by use of a microscope image analysis system. ATP (400 mumol l-1) evoked a prompt increase in [Ca2+]i in all cell preparations in both the presence or absence of extracellular Ca2+ when measured with the cuvette method. Single cell measurements using oviductal cells from cyclic heifers revealed a heterogeneous [Ca2+]i pattern in response to ATP, with some cells either failing to respond or reacting very slowly. Platelet-activating factor (PAF, 30 nmol l-1) induced a rapid increase in [Ca2+]i especially in cells derived from cyclic and pregnant animals, but the effect was significantly less in cells from heifers at oestrus. The increase in [Ca2+]i in bovine cells induced by PAF was reduced when extracellular calcium was depleted, indicating that the effect of PAF on [Ca2+]i involves an influx from the extracellular space. Voltage-sensitive calcium channels do not appear to be involved i...Continue Reading

Citations

Mar 1, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·U TiemannK Wollenhaupt
Nov 26, 2013·Purinergic Signalling·Geoffrey Burnstock
Sep 30, 2006·Human Fertility : Journal of the British Fertility Society·Kai-Fai Lee, William S B Yeung
May 20, 2015·Autonomic Neuroscience : Basic & Clinical·George I Gorodeski
Mar 26, 2005·Human Reproduction Update·Chris O'Neill
Mar 28, 2008·Reproduction in Domestic Animals = Zuchthygiene·U Tiemann
Dec 18, 2001·American Journal of Physiology. Cell Physiology·George I Gorodeski
Aug 24, 1999·Biology of Reproduction·S J DowningH J Leese
Dec 18, 2001·American Journal of Physiology. Cell Physiology·George I Gorodeski
Jun 9, 2020·Molecular Reproduction and Development·Sabine KölleHeather Steele

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