Effect of ATP sulfurylase overexpression in bright yellow 2 tobacco cells. Regulation Of atp sulfurylase and SO4(2-) transport activities

Plant Physiology
Y HatzfeldJ C Davidian

Abstract

To determine if the ATP sulfurylase reaction is a regulatory step for the SO4(2-)-assimilation pathway in plants, an Arabidopsis thaliana ATP sulfurylase cDNA, APS2, was fused to the 35S promoter of the cauliflower mosaic virus and introduced by Agrobacterium tumefaciens-mediated transformation into isolated Bright Yellow 2 tobacco (Nicotiana tabacum) cells. The ATP sulfurylase activity in transgenic cells was 8-fold that in control cells, and was correlated with the expression of a specific polypeptide revealed by western analysis using an anti-ATP sulfurylase antibody. The molecular mass of this polypeptide agreed with that for the overexpressed mature protein. ATP sulfurylase overexpression had no effect on [35S]SO4(2-) influx or ATP sulfurylase activity regulation by S availability, except that ATP sulfurylase activity variations in response to S starvation in transgenic cells were 8 times higher than in the wild type. There were also no differences in cell growth or sensitivity to SeO4(2-) (a toxic SO4(2-) analog) between transgenic and wild-type cells. We propose that in Bright Yellow 2 tobacco cells, the ATP sulfurylase derepression by S deficiency may involve a posttranscriptional mechanism, and that the ATP sulfurylase...Continue Reading

References

Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·Z Reuveny
Oct 1, 1992·Journal of General Microbiology·D ThomasY Surdin-Kerjan
Jan 1, 1990·Annual Review of Biochemistry·W E Rauser
Dec 1, 1973·Analytical Biochemistry·W Schaffner, C Weissmann
Jan 1, 1984·Folia Microbiologica·A AlonsoM A Díaz
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·F W SmithD T Clarkson
Jan 1, 1993·Critical Reviews in Biochemistry and Molecular Biology·T S Leyh
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·O ShaulD Inze
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·A SetyaT Leustek
Feb 1, 1975·Plant Physiology·I K Smith

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Citations

Nov 25, 2005·Photosynthesis Research·T G SorsD E Salt
Jul 17, 2008·Journal of Biomedicine & Biotechnology·Rafael Moreno-SánchezViridiana Olín-Sandoval
Dec 21, 2002·The Plant Journal : for Cell and Molecular Biology·George TsakraklidesThomas Leustek
Nov 4, 2006·Plant, Cell & Environment·Malcolm J Hawkesford, Luit J De Kok
Jun 25, 2013·Bioscience Reports·Geoffrey E RaviliousJoseph M Jez
Aug 17, 2005·FEMS Microbiology Reviews·David Mendoza-CózatlRafael Moreno-Sánchez
Feb 9, 2010·Molecular Plant·Jean-Claude Davidian, Stanislav Kopriva
Jun 9, 2005·The Plant Journal : for Cell and Molecular Biology·Thomas G SorsDavid E Salt
Aug 30, 2005·Journal of Theoretical Biology·David G Mendoza-Cózatl, Rafael Moreno-Sánchez
Mar 25, 2014·Biochimica Et Biophysica Acta·Li ZhangYafei Huang
May 9, 2019·Plant Cell Reports·Alejandro M ArmasDiego F Gomez-Casati
Oct 1, 2005·Functional Plant Biology : FPB·Adamantia AgalouHerman P Spaink
Jul 10, 1999·Plant Physiology·T Leustek, K Saito

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