Effect of atrial natriuretic peptide and calcium antagonists on platelet-activating factor-induced contraction and intracellular calcium mobilization in rat mesangial cells

Journal of Cardiovascular Pharmacology
A OliveraJ M López-Novoa

Abstract

We studied the effect of platelet-activating factor (PAF-acether) on rat mesangial cells (MC) and the intracellular mechanisms for PAF-acether-mediated MC activation. Contraction was measured as changes in planar cell surface area (PCSA), and intracellular free calcium concentration ([Ca2+]i) was measured as changes in the signal of the fluorescent indicator fura-2. PAF-acether induced a time- and dose-dependent reduction in PCSA and a dose-dependent increase in [Ca2+]i. Both phenomena were abolished by preincubation of the cells with the PAF-acether-binding blockers L652,731 and BN 52021. TMB-8, an inhibitor of intracellular calcium mobilization, partially inhibited both the reduction in PCSA and the increase in [Ca2+]i, whereas the Ca2+ entry blocker verapamil completely inhibited the reduction in PCSA but only partially blocked the increase in [Ca2+]i. Atrial natriuretic peptide (ANP) produced a dose-dependent inhibition of PAF-acether-induced cell contraction, but its effect was not statistically related to changes in [Ca2+]i. These results show that PAF-acether-induced MC contraction is associated with transient increases in [Ca2+]i, but the relaxing effects of ANP and verapamil may require additional physiologic interacti...Continue Reading

Citations

May 7, 1999·Kidney International·J M López-Novoa
Feb 7, 2015·The American Journal of Pathology·Anna Reznichenko, Ron Korstanje
Jul 23, 1998·Physiological Reviews·J D Stockand, S C Sansom
Jul 3, 2007·Toxicology and Applied Pharmacology·Carlos Martínez-SalgadoJosé M López-Novoa
Jun 1, 1997·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·J D Stockand, S C Sansom

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