Effect of autolysis on the specificity of bovine spongiform encephalopathy rapid tests.

BMC Research Notes
Daniela MeloniE Bozzetta

Abstract

Routine rapid testing for Bovine Spongiform Encephalopathy (BSE) has highlighted some problems with BSE rapid test performance, the most significant being the number of initially reactive samples and the false positive results on autolyzed tissue. This point is important for BSE active surveillance in risk populations, because tissue autolysis is often unavoidable in routine cases. A robust test suitable for use on field material is therefore needed. To date, very limited information regarding the effect of autolysis on the robustness of rapid tests has been documented; therefore, the National Reference Centre for Animal Encephalopathies (CEA) rapid test laboratory selected 450 autolyzed and negative brain stem samples from fallen stock bovines older than 24 months to assess the specificity of four tests approved for BSE active surveillance: Biorad TeSeE, Enfer TSE version 2.0, Prionics(R) Check LIA, and IDEXX Herd Check BSE Antigen Kit EIA. The samples were graded according to the degree of autolysis and then dissected into five portions, four of which randomly assigned to processing by rapid tests and one to be available for confirmatory Western blot analysis. The specificity of the four systems was 100% for all three grades ...Continue Reading

References

Jun 14, 1991·Science·S B Prusiner
Sep 1, 1993·Research in Veterinary Science·M J StackM Dawson
Jan 1, 1997·Human Molecular Genetics·J Collinge
Nov 29, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S O DebeerA A Bencsik
Dec 28, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Eva MonleónJuan J Badiola
Apr 14, 2005·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Angus WearIndu Patel
Nov 11, 2009·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Elena CarraLorenzo Capucci

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Methods Mentioned

BETA
sandwich immunoassay

Software Mentioned

STATA

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