Effect of azithromycin on the LPS-induced production and secretion of phospholipase A2 in lung cells

Biochimica Et Biophysica Acta
Eirini KitsiouliMarilena E Lekka

Abstract

Azithromycin is a member of macrolides, utilized in the treatment of infections. Independently, these antibiotics also possess anti-inflammatory and immunomodulatory properties. Phospholipase A2 isotypes, which are implicated in the pathophysiology of inflammatory lung disorders, are produced by alveolar macrophages and other lung cells during inflammatory response and can promote lung injury by destructing lung surfactant. The aim of the study was to investigate whether in lung cells azithromycin can inhibit secretory and cytosolic phospholipases A2, (sPLA2) and (cPLA2), respectively, which are induced by an inflammatory trigger. In this respect, we studied the lipopolysaccharide (LPS)-mediated production or secretion of sPLA2 and cPLA2 from A549 cells, a cancer bronchial epithelial cell line, and alveolar macrophages, isolated from bronchoalveolar lavage fluid of ARDS and control patients without cardiopulmonary disease or sepsis. Pre-treatment of cells with azithromycin caused a dose-dependent decrease in the LPS-induced sPLA2-IIA levels in A549 cells. This inhibition was rather due to reduced PLA2G2A mRNA expression and secretion of sPLA2-IIA protein levels, as observed by western blotting and indirect immunofluorescence by...Continue Reading

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Dec 28, 2010·Biochimica Et Biophysica Acta·Eleftheria LetsiouMarilena E Lekka
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Citations

Aug 2, 2015·Chemistry and Physics of Lipids·Manuela DenzPeter Müller
Jul 1, 2017·International Journal of Molecular Sciences·Francesca SpadaroLaura Fantuzzi
Nov 30, 2019·Frontiers in Cellular Neuroscience·Timothy J KopperJohn C Gensel
Jun 10, 2021·Cell Biochemistry and Biophysics·Eleftheria LetsiouSteven M Dudek
Sep 4, 2021·Biomedical Journal·Eirini KitsiouliMarilena E Lekka

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