Abstract
Azithromycin is a member of macrolides, utilized in the treatment of infections. Independently, these antibiotics also possess anti-inflammatory and immunomodulatory properties. Phospholipase A2 isotypes, which are implicated in the pathophysiology of inflammatory lung disorders, are produced by alveolar macrophages and other lung cells during inflammatory response and can promote lung injury by destructing lung surfactant. The aim of the study was to investigate whether in lung cells azithromycin can inhibit secretory and cytosolic phospholipases A2, (sPLA2) and (cPLA2), respectively, which are induced by an inflammatory trigger. In this respect, we studied the lipopolysaccharide (LPS)-mediated production or secretion of sPLA2 and cPLA2 from A549 cells, a cancer bronchial epithelial cell line, and alveolar macrophages, isolated from bronchoalveolar lavage fluid of ARDS and control patients without cardiopulmonary disease or sepsis. Pre-treatment of cells with azithromycin caused a dose-dependent decrease in the LPS-induced sPLA2-IIA levels in A549 cells. This inhibition was rather due to reduced PLA2G2A mRNA expression and secretion of sPLA2-IIA protein levels, as observed by western blotting and indirect immunofluorescence by...Continue Reading
References
May 7, 1976·Analytical Biochemistry·M M Bradford
Sep 1, 1995·Antimicrobial Agents and Chemotherapy·W S Champney, R Burdine
Feb 1, 1997·American Journal of Respiratory and Critical Care Medicine·D L BowtonD A Bass
Jun 18, 1998·The Journal of Antimicrobial Chemotherapy·T R ShryockM Baumholtz
Oct 9, 1999·The Journal of Biological Chemistry·M MurakamiI Kudo
May 9, 2001·Nucleic Acids Research·M W Pfaffl
May 17, 2001·Antimicrobial Agents and Chemotherapy·K TatedaC Van Delden
Jul 11, 2002·Microbes and Infection·Marina A Dobrovolskaia, Stefanie N Vogel
Oct 18, 2002·Lancet·A EquiM Rosenthal
Dec 25, 2002·Antimicrobial Agents and Chemotherapy·Takeshi YamaryoTsuyoshi Nagatake
Feb 12, 2004·Chest·Mark H Gotfried
Feb 12, 2004·Chest·Bruce K Rubin, Markus O Henke
Mar 30, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Shawn J SkerrettChristopher B Wilson
Apr 9, 2005·Critical Care Medicine·George NakosMarilena E Lekka
Jun 21, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Samir MedjaneLhousseine Touqui
Nov 9, 2005·The Journal of Allergy and Clinical Immunology·Massimo TriggianiGianni Marone
Jun 2, 2006·The European Respiratory Journal·S HodgeP N Reynolds
Sep 6, 2006·Biochimica Et Biophysica Acta·Massimo TriggianiGianni Marone
Jan 11, 2007·Antimicrobial Agents and Chemotherapy·Cristina CiganaPaola Melotti
Apr 7, 2007·The Journal of Antimicrobial Chemotherapy·Masaharu ShinkaiBruce K Rubin
Mar 15, 2008·Current Opinion in Pharmacology·Yolanda S López-Boado, Bruce K Rubin
Mar 24, 2009·PloS One·Shibichakravarthy KannanMin Wu
Jul 7, 2009·Biochimica Et Biophysica Acta·Eirini KitsiouliMarilena E Lekka
Jul 6, 2010·Biochimica Et Biophysica Acta·Eleana HatzidakiMarilena E Lekka
Jul 9, 2010·Clinical Microbiology Reviews·Soichiro Kanoh, Bruce K Rubin
Dec 28, 2010·Biochimica Et Biophysica Acta·Eleftheria LetsiouMarilena E Lekka
Jan 5, 2011·International Immunopharmacology·Martina BosnarVesna Eraković Haber
Apr 23, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·A KarkabounasM E Lekka
Jul 7, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Mihailo BanjanacVesna Eraković Haber
Citations
Oct 7, 2015·Journal of Veterinary Pharmacology and Therapeutics·K D DeDonderR K Tessman
Aug 17, 2016·Veterinary Microbiology·Keith D DeDonderRonald K Tessman
Aug 2, 2015·Chemistry and Physics of Lipids·Manuela DenzPeter Müller
Jul 1, 2017·International Journal of Molecular Sciences·Francesca SpadaroLaura Fantuzzi
Nov 30, 2019·Frontiers in Cellular Neuroscience·Timothy J KopperJohn C Gensel
Sep 3, 2020·Chinese Medicine·Nabijan MohammadtursunJingcheng Dong
Jul 12, 2017·Oncology Letters·Fajiu LiChenghong Li
Dec 19, 2020·Experimental Lung Research·Nafiseh NaderiJean Bourbeau
Nov 17, 2020·Frontiers in Cell and Developmental Biology·Yu-Fei ChenXiao-Qing Chen
Jun 10, 2021·Cell Biochemistry and Biophysics·Eleftheria LetsiouSteven M Dudek
Sep 4, 2021·Biomedical Journal·Eirini KitsiouliMarilena E Lekka