PMID: 9176719Jan 1, 1997Paper

Effect of basic fibroblast growth factor on angiogenesis and growth of isografted bone: quantitative in vitro-in vivo analysis in mice

International Journal of Microcirculation, Clinical and Experimental
M LeunigR K Jain

Abstract

Basic fibroblast growth factor (bFGF), a constituent of bone and cartilage matrix, has been shown to be a potent mitogen for osteoblasts and chondrocytes and yet an inhibitor of chondrocyte terminal differentiation in cell culture. To characterize the effect of bFGF on bone formation, whole neonatal murine femora were cultured in the presence or absence of bFGF and a neutralizing antibody against bFGF. In vitro, femoral elongation was provided by cartilage growth only; the calcified diaphyseal zone stained by oxytetracycline did not increase. When bFGF was added to the culture medium, longitudinal growth of the proximal and distal cartilage was inhibited in a dose-dependent manner (p < 0.05), and the number of hypertrophic chondrocytes in the growth plate was reduced. This phenomenon was absent in the presence of a neutralizing antibody, which when given alone significantly promoted femoral elongation. In contrast, in vivo after transplantation into adult mice bearing dorsal skin fold chambers, femora rapidly calcified after revascularization. This observation supports the notion that bone formation largely depends on angiogenesis-mediated events. To verify this hypothesis, angiogenesis and bone formation were quantified using ...Continue Reading

Citations

May 10, 2005·Archives of Oral Biology·M DelatteA M Kuijpers-Jagtman
Nov 10, 2001·Annual Review of Biomedical Engineering·R K Jain
Oct 10, 2012·Bone·Ryan E TomlinsonMatthew J Silva
Jun 9, 2005·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Ruth Board, Gordon C Jayson
Jan 26, 1999·Clinical Orthopaedics and Related Research·J Glowacki

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