Effect of bcl-2 overexpression in mice on ovotoxicity caused by 4-vinylcyclohexene

Toxicology and Applied Pharmacology
Jodi A FlawsPatricia B Hoyer

Abstract

The occupational chemical 4-vinylcyclohexene (VCH) destroys small preantral ovarian follicles in mice following repeated daily dosing. The cell survival gene bcl-2 is thought to protect against follicular death during embryogenesis because primordial follicle numbers in newborn bcl-2 overexpressing (OE) mice are greater than in wild-type (WT) controls. Thus, this study was designed to determine if overexpression of bcl-2 protects against VCH-induced follicle loss during embryonic development. Pregnant bcl-2 OE or WT mice were dosed (p.o.) daily with VCH (500 mg/kg) or sesame oil (vehicle control) on days 8-18 of pregnancy. Ovaries were collected from moms and female pups on pup postnatal day (PND) 8. Nonpregnant OE and WT females were also treated with VCH (500 mg/kg p.o.) or vehicle and evaluated in the same manner. As previously reported, ovaries from PND8 OE female pups contained 50% more primordial follicles than WT pups (P < 0.05). Unlike WT pups, relative to vehicle controls, in utero exposure to VCH resulted in a reduction in primordial (25% of control), primary (38% of control), and secondary (33% of control) follicles in ovaries of OE pups (P < 0.05). VCH had no significant effect on follicle numbers in OE or WT moms. ...Continue Reading

Citations

Dec 15, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·R John AitkenJeff B Kerr
Aug 10, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Jeffrey B KerrRichard A Anderson
Jan 25, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Chuck R GreenfeldJodi A Flaws
Jul 10, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Chuck R GreenfeldJodi A Flaws
May 27, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Michelle MyersKarla J Hutt
Feb 27, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Kavitha VaithiyanathanKarla J Hutt
Nov 26, 2014·Reproduction, Fertility, and Development·Seng H LiewKarla J Hutt

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