Effect of benzo[a]pyrene on P-glycoprotein-mediated transport in Caco-2 cell monolayer

Toxicology
Narumi SugiharaKoji Furuno

Abstract

The main exposure pathway of benzo[a]pyrene (Bap) for humans is considered to be via the daily diet. The purpose of this study was to investigate the effect of BaP on the intestinal transport of chemicals mediated by P-glycoprotein (P-gp). The intestinal epithelial membrane transport of rhodamine-123 (Rho-123), a substrate of P-gp, was examined using a monolayer of the human Caco-2 cell line grown in transwells. In the monolayer exposed to Bap for 72 h before transport experiments, the ratio of the apparent permeability coefficients (P(app)) of Rho-123 efflux increased compared to that of the control. The permeability of rhodamine-B (Rho-B), not a substrate of P-gp, showed no difference between the monolayers. Treatment with quinidine or cyclosporine A, which are P-gp inhibitors, decreased the P(app) of Rho-123 to the same degree in both monolayers. The transport of Rho-123 was not influenced by the presence of Bap. Thus, Bap seemed not to act directly on the efflux activity of P-gp and be a binding site competitor of Rho-123. In the Caco-2 cells that enhanced the efflux of Rho-123 by the treatment with Bap, an increase in mRNA expression of MDR 1 (P-gp) was confirmed compared to that of control by RT-PCR. Furthermore, Western ...Continue Reading

References

Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·F ThiebautM C Willingham
Aug 1, 1997·The European Respiratory Journal·E Lechapt-ZalcmanJ F Bernaudin
Feb 25, 2000·Therapeutic Drug Monitoring·Y Tanigawara
May 17, 2000·European Journal of Pharmacology·Y MitsunagaY Sawada
Oct 21, 2000·European Journal of Pharmacology·Z H HuangM Takano
Apr 12, 2001·The Journal of Biological Chemistry·A GeickO Burk
Jan 18, 2002·Journal of Neurochemistry·Robert A Felix, Margery A Barrand
May 9, 2002·Drug Metabolism Reviews·Richard B Kim
Jun 13, 2002·Environmental Health Perspectives·Carl-Elis BoströmRoger Westerholm
Apr 5, 2003·Toxicology Letters·Adrienn BodóBalázs Sarkadi
Jul 29, 2003·Clinical Pharmacokinetics·Mikko NiemiKari T Kivistö
Dec 4, 2003·European Journal of Clinical Investigation·M F Fromm
May 29, 2004·Therapeutic Drug Monitoring·Scott R PenzakJudith Falloon
Jan 26, 2005·Toxicology and Applied Pharmacology·Annu AroraYogeshwer Shukla

❮ Previous
Next ❯

Citations

Dec 22, 2007·Nanomedicine·Robert M StreicherSilvana Fiorito
Aug 12, 2009·Nanomedicine·Margriet Vdz ParkWim H de Jong
Nov 3, 2010·Nanomedicine·Inmaculada PosadasValentín Ceña
Dec 20, 2008·Nanomedicine·Jean-Pierre KaiserPeter Wick
Apr 1, 2014·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Françoise Vincent-HubertCatherine Gourlay-Francé
Oct 26, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Luba VasilukMargo M Moore
Apr 6, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Narumi SugiharaKoji Furuno
Aug 23, 2008·The Journal of Pharmacy and Pharmacology·Masaaki KadowakiKoji Furuno
May 29, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yu SakutaKiichi Sato

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.