Effect of berberine on LPS-induced expression of NF-κB/MAPK signalling pathway and related inflammatory cytokines in porcine intestinal epithelial cells.

Innate Immunity
Zhang ZhuWang Zili

Abstract

Berberine is an alkaloid extracted from medicinal plants such as Coptis chinensis and Phellodendron chinense. It possesses anti-inflammatory, anti-tumour and anti-oxidation properties, and regulates Glc and lipid metabolism. This study explored the mechanisms of the protective effects of berberine on barrier function and inflammatory damage in porcine intestinal epithelial cells (IPEC-J2) induced by LPS. We first evaluated the effects of berberine and LPS on cell viability. IPEC-J2 cells were treated with 5 μg/ml LPS for 1 h to establish an inflammatory model, and 75, 150 and 250 μg/ml berberine were used in further experiments. The expression of IL-1β, IL-6 and TNF-α was measured by RT-PCR. The key proteins of the NF-κB/MAPK signalling pathway (IκBα, p-IκBα, p65, p-p65, c-Jun N-terminal kinase (JNK), p-JNK, p38, p-p38, ERK1/2 and p-ERK1/2) were detected by Western blot. Upon exposure to LPS, IL-1β, IL-6 and TNF-α mRNA levels and p-IκBα p-p65 protein levels were significantly enhanced. Pre-treatment with berberine reduced the expression of inflammatory factors and was positively correlated with its concentration, and dose dependently inhibited the expression of IκBα, p-IκBα, p-p65, p-p38 and JNK. These results demonstrated that...Continue Reading

References

Jun 6, 2003·Cytokine & Growth Factor Reviews·Klaus Pfeffer
Jun 27, 2003·American Journal of Physiology. Cell Physiology·Laurence J EganMartin F Kagnoff
Mar 26, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jun SunJames L Madara
Apr 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maria T AbreuMoshe Arditi
Dec 13, 2006·Biochimica Et Biophysica Acta·Phillipe Coulombe, Sylvain Meloche
May 15, 2007·Oncogene·A S DhillonW Kolch
Oct 6, 2007·Immunologic Research·Theresa L Gioannini, Jerrold P Weiss
Sep 19, 2008·Comparative Immunology, Microbiology and Infectious Diseases·C ArceJ J Garrido
Feb 13, 2010·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Ning LiJieshou Li
Dec 17, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Fang YanD Brent Polk
Nov 6, 2012·Gene·Shen-Hsi YangAlan J Whitmarsh
Feb 27, 2017·Trends in Cell Biology·Leen Catrysse, Geert van Loo
Apr 3, 2018·Acta histochemica·Ewa GrelaAgnieszka Grabowiecka
Jul 2, 2019·Archivos de bronconeumología·Jaime Eduardo Davino-ChiovattoRodolfo Paula Vieira
Jul 28, 2019·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Ahmed A ElmarakbyMohamed Al-Shabrawey
Dec 11, 2019·European Journal of Medicinal Chemistry·Hamed MortazaviAmir Abbas Momtazi-Borojeni

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protein assay
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