Effect of beta-carotene on cell cycle progression of human fibroblasts

Carcinogenesis
L A StivalaE Prosperi

Abstract

The uptake of beta-carotene (BC) and its effect on the cell cycle progression of normal human fibroblasts in primary culture were investigated by using two different delivery methods: exposure to BC solubilized in the organic solvent tetrahydrofuran (THF) or to BC incorporated into dipalmitoylphosphatidylcholine (DPPC) liposomes. Cell cycle progression was evaluated by immunofluorescence detection and flow cytometric analysis of the proliferating cell nuclear antigen (PCNA). In contrast to THF, which induced a marked reduction in the number of cells in S phase and in the extent of PCNA immunolabeling, DPPC liposomes proved to be an effective delivery system that does not interfere with cell proliferation. Cellular uptake of 0.23 nmol/10(6) cells was found after 24 h incubation in BC-containing DPPC liposomes. This value increased to 1.2 nmol/10(6) cells after 72 h. After the first day of incubation, the number of cells in S phase was reduced by approximately 50%, with a consequent accumulation of cells in G1 phase. This effect was maintained up to 3 days incubation, with no detectable effects on cell viability. This cell cycle delay was found to be reversible, returning the percentage of cells in S phase to the control value 24...Continue Reading

Citations

Aug 12, 2010·Journal of Neural Transmission·Santhrani Thaakur, Ravi Sravanthi
Apr 12, 2000·European Journal of Biochemistry·L A StivalaE Prosperi
Oct 19, 2013·Marine Drugs·Carmen-María López-SaizArmando Burgos-Hernández
May 30, 2001·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·I FrouinA I Scovassi
Dec 20, 2014·International Journal of Molecular Sciences·Carmen-María López-SaizArmando Burgos-Hernández
Oct 2, 2015·Environmental Science and Pollution Research International·Mayada R FaragSahar A Ahmed
Feb 18, 2016·Oxidative Medicine and Cellular Longevity·Samir A E BashandyIbrahim M Alhazza
Aug 21, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Mukesh Kumar SharmaMadhu Kumar
Mar 21, 2007·The Journal of Nutritional Biochemistry·Hsin-Chung ChengShih-Yi Huang
Jan 12, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Mukesh Kumar SharmaMadhu Kumar
Nov 18, 2015·Carbohydrate Polymers·Jong-Yea Kim, Kerry C Huber
Nov 4, 2005·Chemistry and Physics of Lipids·Paola PalozzaNevio Picci
Sep 1, 2005·Environmental Toxicology and Pharmacology·Mukesh Kumar SharmaAshok Kumar
Mar 9, 2002·Toxicology and Applied Pharmacology·Michael GleiBeatrice Louise Pool-Zobel
May 20, 2019·Environmental Science and Pollution Research International·Mohamed HamedAlaa El-Din H Sayed
May 23, 2015·Molecular and Cellular Biochemistry·Poorigali Raghavendra-Rao SowmyaRangaswamy Lakshminarayana

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