Effect of bFGF on invasion of ovarian cancer cells through the regulation of Ets-1 and urokinase-type plasminogen activator

Pharmaceutical Biology
Ting Li, Sen Jiang

Abstract

The aim of the study was to explore the role of basic fibroblast growth factor (bFGF) in ovarian cancer progression. This was done by investigating the effects of bFGF on both the secretion of urokinase-type plasminogen activator (uPA) and the invasion of tumor cells in SKOV(3) ovarian cancer cells. Human ovarian cancer cell line SKOV(3) was cultured in vitro. The expression of uPA gene and protein was induced in SKOV(3) cells; the impact of bFGF on the expression of uPA gene in SKOV(3) cells was studied by RT-PCR, and the impact of bFGF on the expression of uPA protein was tested by ELISA. Ets-1 antisense oligonucleotides were transfected into SKOV(3) cells by liposome protocol. The effects of bFGF on Ets-1 expression and the invasion ability of SKOV(3) cells were determined both before and after exposure to different concentrations of bFGF for 24 h. The expression of both uPA gene and protein was induced in SKOV(3) cells, p < 0.05. The expression of uPA was suppressed by Ets-1 antisense oligonucleotides in SKOV(3) cells, p < 0.05. The invasion ability of SKOV(3) cells was increased by 2.3-fold, and this effect was also suppressed by Ets-1 antisense oligonucleotides. bFGF can enhance the invasion ability of ovarian cancer cell...Continue Reading

References

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Citations

Jul 10, 2012·Computational Statistics & Data Analysis·Shuangge MaYang Xie
May 28, 2011·Reproduction in Domestic Animals = Zuchthygiene·J LinC Zhang
Jun 29, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Kai ZhouTing-Rong Zhang
Mar 17, 2012·Reproductive Sciences·Ya-Min ChengMing-Jenn Chen

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