Effect of Bifunctional β Defensin 2-Modified Scaffold on Bone Defect Reconstruction

ACS Omega
Yiyu PengXiaoping Bi

Abstract

Bone tissue engineering has emerged as an effective alternative treatment to the problem of bone defect. To repair a bone defect, antibiosis and osteogenesis are two essential aspects of the repair process. By searching the literature and performing exploratory experiments, we found that β defensin 2 (BD2), with bifunctional properties of antibiosis and osteogenesis, was a feasible alternative for traditional growth factors. The antimicrobial ability of BD2 against Staphylococcus aureus and Escherichia coli was studied by the spread plate and live/dead staining methods (low effective concentration of 20 ng/mL). BD2 was also demonstrated to enhance osteogenesis, with higher messenger RNA (mRNA) and protein expression of the osteogenic markers collagen I (Col1), runt-related transcription factor 2 (Runx2), osteopontin (Opn), and osteocalcin (Ocn) in vitro (1.5-2.5-fold increase compared with the control group in the most effective concentration group), which was consistent with the alkaline phosphatase (ALP) and alizarin red S (ARS) staining results. We implanted poly(sebacoyl diglyceride) (PSeD) combined with BD2 and rat bone tissue-derived mesenchymal stem cells (rBMSCs) under the back skin of rats and found that the inflammato...Continue Reading

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Citations

Mar 6, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zheng-Yang ChenFang Zhou
Mar 16, 2021·Molecular Oral Microbiology·John Yun NiuChun Hung Chu
May 21, 2021·Frontiers in Bioengineering and Biotechnology·Lunhao LiXiaoping Bi

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Methods Mentioned

BETA
electrophoresis
dissection
X-ray

Software Mentioned

Image
Pro
MicroView
SPSS

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