Effect of bioaugmentation and biostimulation on sulfate-reducing column startup captured by functional gene profiling

FEMS Microbiology Ecology
L P PereyraAmy Pruden

Abstract

Sulfate-reducing permeable reactive zones (SR-PRZs) depend upon a complex microbial community to utilize a lignocellulosic substrate and produce sulfides, which remediate mine drainage by binding heavy metals. To gain insight into the impact of the microbial community composition on the startup time and pseudo-steady-state performance, functional genes corresponding to cellulose-degrading (CD), fermentative, sulfate-reducing, and methanogenic microorganisms were characterized in columns simulating SR-PRZs using quantitative polymerase chain reaction (qPCR) and denaturing gradient gel electrophoresis (DGGE). Duplicate columns were bioaugmented with sulfate-reducing or CD bacteria or biostimulated with ethanol or carboxymethyl cellulose and compared with baseline dairy manure inoculum and uninoculated controls. Sulfate removal began after ~ 15 days for all columns and pseudo-steady state was achieved by Day 30. Despite similar performance, DGGE profiles of 16S rRNA gene and functional genes at pseudo-steady state were distinct among the column treatments, suggesting the potential to control ultimate microbial community composition via bioaugmentation and biostimulation. qPCR revealed enrichment of functional genes in all columns ...Continue Reading

References

Nov 1, 1989·Applied and Environmental Microbiology·T G LinkfieldJ M Tiedje
Oct 31, 2000·Applied and Environmental Microbiology·M T SuzukiE F DeLong
Jan 19, 2002·Ground Water·Ralph D LudwigKeith Mountjoy
Apr 12, 2002·Environmental Science & Technology·K R WaybrantD W Blowes
May 3, 2002·Journal of Advanced Nursing·Kathleen C Insel, Terry A Badger
Sep 25, 2002·Environmental Science & Technology·Lesley C Batty, Paul L Younger
Sep 23, 2003·Research in Microbiology·D Barrie Johnson, Kevin B Hallberg
Dec 31, 2003·International Journal of Pharmaceutics·Mirka KorhonenJouko Yliruusi
Feb 1, 2005·The Science of the Total Environment·D Barrie Johnson, Kevin B Hallberg
Feb 1, 2005·The Science of the Total Environment·D Barrie Johnson, Kevin B Hallberg
Jan 10, 2006·Microbial Ecology·Duongruitai NicomratOlli H Tuovinen
Jan 19, 2007·Journal of Microbiological Methods·Hyunsuk HongKenneth F Reardon
Dec 21, 2007·Journal of Hazardous Materials·Aijie WangDuujong Lee
Jul 9, 2008·Environmental Science & Technology·Santosh R MohantyEric E Roden
Jul 16, 2008·Journal of Microbiological Methods·Kathrin Euringer, Tillmann Lueders

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioremediation (ASM)

Bioremediation is the treatment and removal of harmful pollutants or contaminants through the use of microorganisms. Discover the latest research here.