Effect of biocontrol agent Pseudomonas fluorescens 2P24 on soil fungal community in cucumber rhizosphere using T-RFLP and DGGE.

PloS One
Guanpeng GaoWei Wang

Abstract

Fungi and fungal community play important roles in the soil ecosystem, and the diversity of fungal community could act as natural antagonists of various plant pathogens. Biological control is a promising method to protect plants as chemical pesticides may cause environment pollution. Pseudomonas fluorescens 2P24 had strong inhibitory on Rastonia solanacearum, Fusarium oxysporum and Rhizoctonia solani, etc., and was isolated from the wheat rhizosphere take-all decline soils in Shandong province, China. However, its potential effect on soil fungal community was still unknown. In this study, the gfp-labeled P. fluorescens 2P24 was inoculated into cucumber rhizosphere, and the survival of 2P24 was monitored weekly. The amount decreased from 10(8) to 10(5) CFU/g dry soils. The effect of 2P24 on soil fungal community in cucumber rhizosphere was investigated using T-RFLP and DGGE. In T-RFLP analysis, principle component analysis showed that the soil fungal community was greatly influenced at first, digested with restriction enzyme Hinf I and Taq I. However, there was little difference as digested by different enzymes. DGGE results demonstrated that the soil fungal community was greatly shocked at the beginning, but it recovered slowly...Continue Reading

References

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Citations

Aug 21, 2013·BioMed Research International·Darine Trabelsi, Ridha Mhamdi
Oct 15, 2013·Applied and Environmental Microbiology·Zhiyuan YaoJianming Xu
Sep 12, 2013·Phytopathology·Justine SyllaWalter Wohanka
Dec 3, 2014·Journal of Environmental Sciences (China)·Liezhong ChenYunlong Yu
Oct 6, 2020·Frontiers in Microbiology·Costantino VischettiEdoardo Puglisi
May 10, 2018·Scientific Reports·Tal HassanovIlana Kolodkin-Gal
Sep 18, 2021·Frontiers in Microbiology·Andrea ManfrediniLoredana Canfora

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Methods Mentioned

BETA
Electrophoresis
PCR
PCA

Software Mentioned

SPSS
Statistical Product and Service Solutions
VisionWorksLS
GeneScan Analytical

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