Effect of biopsies on sensitivity and specificity of ultra-rapid urease test for detection of Helicobacter pylori infection: a prospective evaluation

World Journal of Gastroenterology : WJG
Li-Lin LimManuel Salto-Tellez

Abstract

To prospectively assess the sensitivity, specificity and time to positivity of theUltra-rapid urease test (URUT) for Helicobacter pylori (H pylori), and compare the results of one with those of two biopsies. Five antral biopsies were taken in consecutive patients undergoing upper endoscopy: one and two biopsies for URUT, and one each for H pylori culture and histology. URUT was read at 1, 5, 10, 20 and 30 min, 1, 2, 3 and 24 h after biopsy insertion into the reagent. A positive histology and/or culture was used as positive reference "gold standards". URUT was more sensitive for detecting H pylori with two biopsies rather than one, at all time points up to 120 min. The sensitivity improved from 3.6% to 82.1% for one biopsy and 10.7% to 85.7% for two biopsies from 1 to 120 min. The sensitivity reached 96.4% at 24 h for both, but the specificity reduced from 100% to 96% and 92% for one and two biopsies, respectively. Development of a positive URUT result is hastened by doubling the number of gastric biopsies. We recommend taking two instead of one biopsy to achieve an earlier positive URUT result so that H pylori eradication therapy can be initiated before patient is discharged from the endoscopy suite.

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Citations

Sep 16, 2008·Best Practice & Research. Clinical Gastroenterology·Ralf KiesslichMarkus F Neurath
Aug 5, 2008·Gastrointestinal Endoscopy Clinics of North America·Ralf KiesslichMarkus F Neurath
Dec 20, 2012·Clinical Endoscopy·Il Ju Choi
Apr 14, 2012·Journal of Gastroenterology and Hepatology·Su Jin JeonJin Hong Kim
Nov 25, 2011·The Korean journal of gastroenterology = Taehan Sohwagi Hakhoe chi·Sunyoung NaGeum Ha Kim
Dec 7, 2016·British Journal of Biomedical Science·Najmiyatul FadilahMd Mostafizur Rahman

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