Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells

Scientific Reports
Mythily GanapathiRandall H Morse

Abstract

The ability of isolated neural stem cells (NSCs) to proliferate as neurospheres is indicative of their competence as stem cells, and depends critically on the polycomb group (PcG) member Bmi1: knockdown of Bmi1 results in defective proliferation and self-renewal of isolated NSCs, whereas overexpression of Bmi1 enhances these properties. Here we report genome-wide changes in gene expression in embryonic and adult NSCs (eNSCs and aNSCs) caused by overexpression of Bmi1. We find that genes whose expression is altered by perturbations in Bmi1 levels in NSCs are mostly distinct from those affected in other multipotent stem/progenitor cells, such as those from liver and lung, aside from a small core of common targets that is enriched for genes associated with cell migration and mobility. We also show that genes differing in expression between prospectively isolated quiescent and activated NSCs are not affected by Bmi1 overexpression. In contrast, a comparison of genes showing altered expression upon Bmi1 overexpression in eNSCs and in aNSCs reveals considerable overlap, in spite of their different provenances in the brain and their differing developmental programs.

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Citations

Apr 5, 2019·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·J WangX Yu
Oct 15, 2019·Journal of Cellular Physiology·Divya Desai, Prasad Pethe
Jul 23, 2020·Stem Cells International·Zhenzhen HanWei Li
Jul 18, 2018·Cells·Elkin Navarro QuirozGustavo Aroca Martinez
Oct 22, 2020·Biotechnology and Applied Biochemistry·Mona PourjafarRezvan Najafi
Mar 1, 2021·Life Sciences·Arnab SarkarTapan Kumar Maity
Jul 29, 2021·NAR Cancer·Verónica Freire-BenéitezSilvia Marino

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Datasets Mentioned

BETA
E-MTAB-3491

Methods Mentioned

BETA
RNA-seq
Chip
Hi-Seq

Software Mentioned

Galaxy
Tophat
Cellprofiler
Cufflinks
Cistrome
Generic Gene Ontology Term Finder
Genespring
Cuffdiff
Affymetrix GeneChip Command Console

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