Abstract
In this study, we investigated whether Bone Morphogenetic Protein-2 (BMP-2) could modulate dedifferentiation, apoptosis and proliferation capacity in the normal and OA cultured chondrocytes. The articular chondrocytes from normal (n = 4) and OA (n = 4) cartilages were harvested separately. The chondrocytes were cultured in monolayer in the presence of 100 ng mL(-1) BMP-2 and 1% FBS as a test group and 1% FBS alone as a control group. Then, the chondrocytes were harvested and assessed for morphology with invert microscopy, proliferation by using MTT-assay and apoptosis with caspase-3 immunocytochemistry. The results indicated that the normal and the most OA chondrocytes showed round and polygonal appearance with chondrocyte-like morphology in BMP-2 treated groups after 6 days. The MTT proliferation test didn't show significant difference between test and control groups. The OA cells showed proliferation rate higher than the normal cells and significant difference in the presence of BMP-2 was observed (p<0.05). Positive immunostaining of caspase-3 in test and control groups was 1 and 20% in normal and 30 and 43% in OA groups, respectively. The percentage of apoptosis was reduced in the presence of BMP-2. In conclusion, it appears...Continue Reading
References
Dec 16, 1983·Journal of Immunological Methods·T Mosmann
Mar 4, 1998·Arthritis and Rheumatism·F J BlancoF Galdo
Aug 12, 1999·Zeitschrift für Rheumatologie·M SittingerG R Burmester
Jan 26, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M C StewartJ N Macleod
Aug 16, 2000·Clinical Orthopaedics and Related Research·M UchinoM Itoman
Nov 23, 2000·Annals of the Rheumatic Diseases·F HéraudM F Harmand
Mar 15, 2001·Annals of the Rheumatic Diseases·C JorgensenJ Sany
Dec 6, 2001·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·A LindahlL Peterson
Feb 16, 2002·Cellular and Molecular Life Sciences : CMLS·A A Cole, K E Kuettner
Feb 16, 2002·Cellular and Molecular Life Sciences : CMLS·E Hedbom, H J Häuselmann
Sep 13, 2002·Matrix Biology : Journal of the International Society for Matrix Biology·Beatrice DozinAntonello Pietrangelo
Jun 14, 2003·FEBS Letters·Ulrich ValcourtFrédéric Mallein-Gerin
Aug 20, 2003·The Journal of Bone and Joint Surgery. American Volume·Naoshi FukuiLinda J Sandell
Dec 1, 1960·Annals of the Rheumatic Diseases·D H COLLINS, T F McELLIGOTT
Jun 9, 2004·Biomaterials·K R BrodkinM E Levenston
Jun 29, 2004·Osteoarthritis and Cartilage·Tatiana GründerWilhelm K Aicher
Sep 9, 2005·The Laryngoscope·Jeremy D RichmonDeborah Watson
Sep 12, 2007·Veterinary and Comparative Orthopaedics and Traumatology : V.C.O.T·A O Oshin, M C Stewart