Effect of bone morphogenetic protein-2 on proliferation and apoptosis of gastric cancer cells.

International Journal of Medical Sciences
Junjie ZhangZhirong Wang

Abstract

To investigate the effects of bone morphogenetic protein-2 (BMP-2) on the proliferation, differentiation and apoptosis of normal human gastric mucosal cells and gastric cancer cells. Poorly differentiated gastric cancer BGC823 cells, moderately differentiated gastric cancer cells and normal human gastric mucosal epithelial GES-1 cells were independently treated with recombinant human BMP-2 or its inhibitor Noggin. MTT assay was performed to detect the proliferation, flow cytometry done to measure the cell cycle and apoptosis and immunohistochemistry carried out to determine the expression of cyclin-dependent kinase 4 (CDK4). BMP-2 exerted inhibitory effect on the growth of all types of cells and the inhibition become more evident with the increase of BMP-2 dose. After treatment with 200 ng/ml BMP-2, cancer cells arrested in G1 phase and those in S phase reduced. Gastric cancer cells had higher CDK4 expression than GES-1 cells. BMP-2 decreased CDK-4 expression in cancer cells but had no influence in GES-1 cells. Noggin conferred promotive effect on the growth of 3 types of cells. In 2 types of cancer cells, treatment with 2000 ng/ml Noggin significantly increased the proportion of cells in S phase but reduced that in G1 phase. H...Continue Reading

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Citations

Nov 28, 2012·Journal of Gastroenterology·Guihua XuXiulan Su
Jan 15, 2014·The Spine Journal : Official Journal of the North American Spine Society·Chadi A Tannoury, Howard S An
May 26, 2012·Journal of Cellular Biochemistry·Sharon L HyzyBarbara D Boyan
Oct 18, 2016·Journal of Cellular Biochemistry·Fernanda WisnieskiMarília Cardoso Smith
Jul 31, 2019·Statistics in Medicine·Mengyun Wu, Shuangge Ma
Aug 15, 2015·Spine·Branko SkovrljSamuel K Cho
Mar 28, 2017·Pharmaceutical Research·Inas El BialyM Reza Nejadnik
Jan 29, 2020·Computational and Structural Biotechnology Journal·Zhiwei SunLin Ye

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

SPSS

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