Effect of bone proteins on human prostate cancer cell lines in vitro

The Prostate
T G HullingerM J Somerman

Abstract

Despite the high incidence and serious consequences of skeletal metastasis in prostate cancer patients, the mechanisms involved in establishing secondary lesions in bone are not well-understood. In this study, the role of the mineralized bone matrix in the process of skeletal metastasis was evaluated. Attachment, migration, and proliferation responses of human prostate cancer cells to a crude bone protein extract (CBE) were studied. LNCaP and DU145 cells were utilized in 24-hr attachment assays. Boyden chamber chemotactic assays and cell proliferation assays utilized DU145 cells. CBE and fibronectin (FN) promoted attachment of DU145 cells, whereas only FN facilitated attachment of LNCaP cells. CBE-mediated adhesion of DU145 cells was reduced by 94% with cycloheximide, by 98% with RGD peptides, and by 94% with an antibody to alphavbeta3. Although DU145 cells migrated toward FN, CBE did not promote migration of DU145 cells. DU145 cells grown in the presence of CBE-containing media demonstrated a significant reduction in cell number by day 4. The antiproliferative effect of CBE was not due to cell toxicity. In conclusion, results from this study indicate that mineralized bone proteins promote the attachment of DU145 cells in vitro...Continue Reading

References

Mar 15, 1978·International Journal of Cancer. Journal International Du Cancer·K R StoneD F Paulson
Jun 1, 1989·The Anatomical Record·P V Hauschka, F H Wians
Jul 1, 1989·The Journal of Clinical Investigation·M Chackal-RoyB R Zetter
Jul 1, 1988·Calcified Tissue International·M J SomermanJ J Sauk
Apr 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·W J Grzesik, P G Robey
Jun 1, 1995·Journal of Periodontology·Y ShigeyamaM J Somerman
Jan 1, 1993·Journal of Cancer Research and Clinical Oncology·C M WitkowskiA E Cress
Jan 1, 1994·Breast Cancer Research and Treatment·T YonedaG R Mundy
Feb 1, 1994·Journal of Cellular Biochemistry·J W XuanA F Chambers
Jun 1, 1996·The Journal of Cell Biology·K E Miller, H C Joshi
Jun 1, 1996·Endocrine Reviews·J E MeredithM A Schwartz
Jul 1, 1996·Cell Biology International·M PistoneP Manduca

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Citations

Sep 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Gregory R Mundy
Jan 9, 2004·Reproductive Biology and Endocrinology : RB&E·Delisha A StewartRobert A Sikes
Mar 3, 2004·Journal of Cellular Biochemistry·Kwanchanit TantivejkulKenneth J Pienta
Apr 1, 2005·Neoplasia : an International Journal for Oncology Research·Izabela PodgorskiBonnie F Sloane
Jul 18, 2000·Cancer·F W OrrG Singh
Jun 22, 2000·Journal of Cellular Biochemistry·T G HullingerM J Somerman
Apr 27, 2007·Current Urology Reports·Bulent Akduman, E David Crawford
Jun 13, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Fabian GrafChristian Maercker

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