Effect of bovine serum on the phase transition temperature of cholesterol-containing liposomes

Journal of Microencapsulation
M H Gaber

Abstract

The phase transition temperature of liposomes composed of dipalmitoylphosphatidylcholine (DPPC)/hydrogenated soy phosphatidylcholine (HSPC) at a 2:1 molar ratio was estimated in buffer, 30% and 50% bovine serum by monitoring the leakage of encapsulated self quenched doxorubicin (Dox) from the vesicles when exposed to a temperature increasing from 30-52 degrees C. The results showed that bovine serum caused a slight decrease in the phase transition temperature from 44 to 41 degrees C in 50% serum. Addition of 50% cholesterol to this liposomal composition resulted in the disappearance of transition temperature in buffer and 30% serum, whereas 50% bovine serum resulted in the reappearance of the transition temperature at 46 degrees C. The data suggest that bovine serum affects the transition temperature of liposomes in a concentration-dependent manner, and this effect is more pronounced in cholesterol-rich liposomes. The time course for the release of Dox from both kinds of liposomes (cholesterol rich and cholesterol free) during incubation in 50% bovine serum, at temperatures close to the transition temperature (42 degrees, 45 degrees C), was followed. The results showed an increased leakage of Dox from both kinds of liposomes, a...Continue Reading

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Citations

Jan 25, 2008·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Jessica A TashjianBenjamin L Viglianti
Oct 16, 1999·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·G Kong, M W Dewhirst
Apr 23, 2013·Drug Development and Industrial Pharmacy·Jun ChenBao-chang Cai
May 25, 2002·Drug Development and Industrial Pharmacy·Moses O Oyewumi, Russell J Mumper
Nov 18, 1998·Journal of Microencapsulation
Sep 5, 2017·Journal of Microencapsulation·Quan ZhangShucan Luo
Oct 7, 2010·Nano Letters·Kevin BraeckmansStefaan C De Smedt

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