PMID: 16536980Mar 16, 2006Paper

Effect of brain-derived neurotrophic factor on in vitro metastasis of hepatocellular carcinoma cell line HepG2

Ai zheng = Aizheng = Chinese journal of cancer
Zhen-Yu XiaoXiaoping Chen

Abstract

Metastasis is one of the important reasons for treatment failure of hepatocellular carcinoma (HCC). It is related to biologic behaviors of HCC, including proliferation, migration, and invasion. Recently, researchers have realized that brain-derived neurotrophic factor (BDNF) is a functional protein that exists in HCC tissue, and closely relates to the development of HCC, but the exact mechanism is still unknown. This study was to explore the effect of BDNF on in vitro metastasis of HCC cell line HepG2, and investigate its mechanism. The effect of BDNF on the proliferation of HepG2 cells was examined by MTT assay. The effect of BDNF on the metastasis of HepG2 cells was evaluated by tumor cell migration and invasion assays. The expression of TrkB, a specific receptor of BDNF, in HepG2 cells was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. BDNF efficiently stimulated the proliferation of HepG2 cells in a dose-dependent manner under the concentration of 100 ng/ml. The migration index was significantly higher in the cells treated with 50 or 100 ng/ml BDNF than in control cells (167 and 203 vs. 100, P<0.05). Cell number on the down-side of transwell membrane was significantly higher in the ce...Continue Reading

Related Concepts

Related Feeds

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.

Related Papers

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology
Hong PengJun Hou
Nihon rinsho. Japanese journal of clinical medicine
Masatake KuritaNorimichi Nakahata
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology
Yan XieCheng-Wei Tang
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology
Yuan-e LianAi-min Huang
© 2021 Meta ULC. All rights reserved