Effect of BRCA1 on the Concurrent Chemoradiotherapy Resistance of Cervical Squamous Cell Carcinoma Based on Transcriptome Sequencing Analysis.

BioMed Research International
Xin WenManhua Cui

Abstract

Cervical squamous cell carcinoma (CSCC) is the main pathological type of cervical cancer, accounting for 80%-85% of cervical cancer. Owing to concurrent chemoradiotherapy (CCRT) resistance in a subset of CSCC patients, the treatment response is often unsatisfactory. Identifying predictors and therapeutic targets related to cisplatin-based CCRT resistance in CSCC is critical. We reanalyzed GSE56363, an mRNA dataset from the GEO database with 21 patients with locally advanced CSCC, to identify differentially expressed genes (DEGs) related to CCRT resistance. The hub genes were screened from the protein-protein interaction network of DEGs using cytoHubba plug-in of Cytoscape software. Transcriptome sequencing technology was used to compare differential expression between SiHa cells overexpressing BRCA1 compared with control SiHa cells. Functional annotation for DEGs and gene set enrichment analysis (GSEA) was performed to identify DEG-enriched relative signaling pathways to examine the molecular mechanisms of BRCA1 in CCRT resistance of CSCC. qPCR was used to verify the expression of key genes in SiHa/DDP cells. A total of 609 DEGs including 223 upregulated DEGs and 386 downregulated DEGs were identified between the complete respo...Continue Reading

References

May 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·T OuchiC M Horvath
Oct 18, 2002·Lancet·Richard D KennedyD Paul Harkin
Nov 18, 2004·Journal of the National Cancer Institute·Richard D KennedyD Paul Harkin
Oct 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aravind SubramanianJill P Mesirov
Mar 22, 2007·Molecular Cancer Research : MCR·Niamh E BuckleyPaul B Mullan
Aug 21, 2007·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Karin SöderlundMarie Stenmark Askmalm
Nov 28, 2008·International Journal of Cancer. Journal International Du Cancer·Johanne WeberpalsJim Dimitroulakos
Mar 25, 2009·American Journal of Clinical Oncology·Alain FourquetUNKNOWN Institut Curie Breast Ovary Cancer Risk Study Group
Apr 8, 2010·Chemico-biological Interactions·Jessica M HairAlexandros G Georgakilas
Mar 19, 2011·BMC Bioinformatics·Xavier RobinMarkus Müller
Mar 30, 2012·Omics : a Journal of Integrative Biology·Guangchuang YuQing-Yu He
Jul 28, 2012·Trends in Biochemical Sciences·Mischa L Li, Roger A Greenberg
Oct 31, 2013·Journal of the National Cancer Institute·Chaoyang SunGang Chen
Mar 29, 2014·Science·Ashok R Venkitaraman
Aug 12, 2014·Trends in Genetics : TIG·Erwin L van DijkClaude Thermes
Oct 30, 2014·Nucleic Acids Research·Damian SzklarczykChristian von Mering
Dec 19, 2014·BMC Systems Biology·Chia-Hao ChinChung-Yen Lin
Jan 22, 2015·Nucleic Acids Research·Matthew E RitchieGordon K Smyth
Jan 19, 2016·Nature Reviews. Cancer·Christopher J Lord, Alan Ashworth
Jun 29, 2016·Cytokine·Jan PencikLukas Kenner
Jul 23, 2016·Nature·Arnab Ray ChaudhuriAndré Nussenzweig
May 4, 2017·Cancer·William SmallDavid K Gaffney
Jun 4, 2017·Molecular and Cellular Endocrinology·Bernd Groner, Viktoria von Manstein
Jun 27, 2018·Trends in Genetics : TIG·Erwin L van DijkClaude Thermes
Jul 20, 2018·European Review for Medical and Pharmacological Sciences·L-L Huang, W Rao
May 6, 2019·Virchows Archiv : an International Journal of Pathology·Dan JiangKun Li

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE56363
GSE141558

Methods Mentioned

BETA
RNA Assay
PCR
transfection

Software Mentioned

limma
GraphPad Prism
Broad
GraphPad
pheatmap
DESeq R package
GSEA
Cytoscape
CytoHubba
clusterProfiler

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.