Effect of bronchoconstrictive aerosols on pulmonary gas trapping in the A/J mouse

Respiration Physiology
C A YiamouyiannisS A Silbaugh

Abstract

We exposed A/J mice to several challenge aerosols and measured gas trapped within excised lungs by quantitating their buoyancy in saline (Archimedes' principle). The temporal stability of the excised lung gas volume (ELGV) measurement was also examined. ELGV increased in a dose proportional manner with increasing concentrations of methacholine and reached a maximum of 338 +/- 33% above vehicle-exposed controls. The A/J mice were 100 times more responsive to aerosol methacholine compared to hyporesponsive C3H/HeJ mice. Aerosol challenges of U-46619, a thromboxane A2 mimetic, and serotonin resulted in a 40% and 135% increase in ELGV's versus their controls, respectively. ELGV's were not increased after aerosols of leukotriene C4, histamine, substance P, N-formyl-methionyl-leucyl-phenyl-alanine and platelet activating factor. Both normal (filtered air-exposed) and hyperinflated (methacholine-exposed) excised lungs lost about 10% of their initial volume by 30 min and 40-65% of initial volume by 4 h. Occlusion of the trachea in either group did not affect the total gas lost, suggesting that majority of the gas loss was via transpleural diffusion. We conclude that determination of ELGV in mice, when performed soon after challenge tes...Continue Reading

References

May 15, 1992·Biochemical and Biophysical Research Communications·T NambaS Narumiya
Jul 1, 1991·The American Review of Respiratory Disease·L W Barger, J N Evans
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Feb 1, 1986·Respiration Physiology·P W Stengel, S A Silbaugh
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Jan 1, 1993·The American Review of Respiratory Disease·P W StengelS A Silbaugh
Nov 1, 1993·Thorax·D F Rogers, B J O'Connor

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Citations

May 24, 2005·Respiratory Physiology & Neurobiology·Anne-Helene JanssonXiangdong Wang
Dec 27, 2011·Free Radical Biology & Medicine·Erin N Potts-KantJohn W Hollingsworth
May 9, 2008·Pulmonary Pharmacology & Therapeutics·Brendan J Canning, Yangling Chou

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