Effect of Buddleja officinalis on high-glucose-induced vascular inflammation in human umbilical vein endothelial cells

Experimental Biology and Medicine
Yun Jung LeeHo Sub Lee

Abstract

In this study, we aimed to investigate whether an aqueous extract of Buddleja officinalis (ABO) suppresses high-glucose-induced vascular inflammatory processes in the primary cultured human umbilical vein endothelial cells (HUVEC). The high-glucose-induced increase in expression of cell adhesion molecules (CAMs) such as intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and endothelial-selectin (E-selectin) was significantly attenuated by pretreatment with ABO in a dose-dependent manner. Enhanced cell adhesion caused by high glucose in co-cultured U937 and HUVEC was also blocked by pretreatment with ABO. Pretreatment with ABO also blocked formation of high-glucose-induced reactive oxygen species (ROS). In addition, ABO suppressed the transcriptional activity of NF-kappaB and IkappaB phosphorylation under high-glucose conditions. Pretreatment with N(G)-nitro-l-arginine methyl ester (L-NAME), an endothelial nitric oxide (NO) synthase inhibitor, attenuated the protective action of ABO on high-glucose-induced CAM expression, suggesting a potential role of NO signaling. The present data suggest that ABO could suppress high-glucose-induced vascular inflammatory processes, and ABO may be closely re...Continue Reading

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Citations

Feb 9, 2012·International Journal of Molecular Sciences·Sun Mi HwangHo Sub Lee
Mar 13, 2014·Chinese Journal of Integrative Medicine·Wei LiYue-hua Jiang
Jan 24, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Rainer W Bussmann
Aug 15, 2015·Biochemical and Biophysical Research Communications·Tadamitsu TsuneyoshiNaoaki Morihara
May 27, 2010·The American Journal of Chinese Medicine·Yun Jung LeeHo Sub Lee
Dec 15, 2010·Experimental Biology and Medicine·Manju S Devi, Perumana R Sudhakaran
Oct 31, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Xiaoteng YanPeirong Lu
Mar 22, 2012·Organic Letters·Zhengxin CaiMichael Harmata
Oct 13, 2011·Journal of Medicinal Chemistry·Jianzhang WuGuang Liang

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Methods Mentioned

BETA
Enzyme Linked Immunosorbent Assay
Assay
fluorescence microscopy
electrophoresis
Transfection
transfections
ELISA

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